WIDAL Testt
اختبار الحمى التيفودية واعراضها ارتفاع فى درجات الحرارة والقىء والتهاب الشعب الهوائية ويمكن ان تسبب تليف فى الكبد .
الكائن المسبب لها يسمى Salmonella sp
Procedure:
The reagents must have the room temp. before using.
Prepare white slide for using .
Put 20 µ of the reagent A , B , O & H on the white slide separating each other by enough space then put 10 µ serum at each reagent zone
Mix each reagent with it's neighbor serum quickly begin rotate the 4 reagents together for 1 min.
The result depends on each reagentبمعنى ان كل كاشف على حدة حيث يمكن واحد او اكثر يعطى ايجابى والاخرين سلبى فبالتالى نكمل فى الايجابى بنظام التتر والسلبى تسجل نتيجته
If -ve so result have titre 1/40.
If +ve (ppt occur) so the titre 1/80 and repeat by dilution the serum with saline by ratio 1 : 1. Put 10 µ of serum mix 1 with 20 µ of reagents that gives +ve result only by the same steps.
If -ve so result 1/80
If +ve so the titre 1/160 and repeat by the same steps of dilution.
The titre of this test is :
1/40 , 1/80 , 1/160 , 1/320 , 1/640
The diagnostic titre is 1/160 .
ASOT
Anti Streptolysin-O-Titre
- نوع من انواع البكتريا من فصيلة streptococcus يصيب اللوزتين .
- تسبب صديد على اللوزتين وزيادتها تصل الى القلب وتسبب مشكله فى عضلة القلب .
***الاعراض :
-- المريض يكون اكثر عرضة للانفلوانزا بالاضافة الى الم فى المفاصل وخاصة الركبة والظهر وعدم القدرة على السير لمسافات طويلة وهذا يحدث غالبا عند الاطفال والشباب .
Procedure :
-->Reagent must have the room temp. before use then mix gently .
1.Put 10 µ of serum in a black slide then put on it 10 µ of the reagent .
2.Mix and rotate the slide by hands in a circle direction for 2 min .
-If precipitation occurs the result + ve. and give titre 200 IU / ml .
-If no ppt occurs the result is -ve .
-In +ve case we must repeat by the following steps:
1.Serum diluted by saline by the ratio 1 : 1 .
2.Take 10 µ from mix and put on the black slide then put 10 µ reagent mix and rotate for 2 min.
-If -ve so the titre 200 IU /ml .
-If +ve the titre 400 IU /ml . and repeat by the dilution of last mix by the ratio 1 : 1 or dilute the serum directly by The ratio 1 serum : 2 saline .and so on till the result give -ve .
The Titre in this test 200 - 400 - 600 - 800 IU/ml and no higher than 800 IU/ml .
IMPORTANT COMMENT:
-->False +ve result may occur in early infection and children between 6 months to 2 years .
RF
Rheumatoid Factor
هذا التحليل يكشف عن مرض الروماتيزم وذا المرض يوصف بانه auto immune disease
Procedure :
(As CRP steps) take 10 µ serum with 10 µ reagent of RF on black slide then rotate by hands for 3 min.
if -ve the result -ve .
if +ve the titre 8 mg/dl and repeat by dilution of serum with saline by ratio 1 : 1 [mix1].
10 µ mix1 with 10 µ reagent rotate for 3 min.
if -ve the result 8 mg/dl.
if +ve the titre 16 mg/dl and repeat by dilution of mix 1 by saline by ratio 1 : 1 and so on.
the titre of this test 8 , 16 , 32 , 64 , 128 , 256 .
IMPORTANT COMMENT
The test can be +ve with persons suffering from syphilis OR hepatitis C
CRP
C-Reactive Protein
هذا التحليل يكشف عن الالتهابات فى الدم .
Procedure:
-10µ serum + 10 µ reagent of CRF (على الشريحة السوداء) then mix with rotation by hands for 3 min.
If -ve so the result is -ve in report
If +ve the titre is 6 mg/dl and we will repeat by the following values:
-The serum diluted by saline by the ratio 1 : 1 [mix1] .
Then take 10 µ of mix with 10 µ of reagent and rotate for 3 min.
If -ve so the result 6 mg/dl .
If +ve so the titre 12 mg/dl and repeat by the following values :
-The mix will diluted by saline by the ratio 1 : 1 or the serum diluted by saline by ratio
1 serum : 2 saline [mix2].
Then take 10 µ of mix2 with 10 µ reagent and rotate for 3 min.
If -ve so the result 12 mg/dl .
If +ve so the titre 24 mg/dl and repeat by the same steps.
-Titre of this test : 6 , 12 , 24 , 48
If the result leads to more than 48 the report is more than 48 and not higher titre values .
IMPORTANT COMMENT :
When testing new born children false -ve result are possible .
اختبار الحمى التيفودية واعراضها ارتفاع فى درجات الحرارة والقىء والتهاب الشعب الهوائية ويمكن ان تسبب تليف فى الكبد .
الكائن المسبب لها يسمى Salmonella sp
Procedure:
The reagents must have the room temp. before using.
Prepare white slide for using .
Put 20 µ of the reagent A , B , O & H on the white slide separating each other by enough space then put 10 µ serum at each reagent zone
Mix each reagent with it's neighbor serum quickly begin rotate the 4 reagents together for 1 min.
The result depends on each reagentبمعنى ان كل كاشف على حدة حيث يمكن واحد او اكثر يعطى ايجابى والاخرين سلبى فبالتالى نكمل فى الايجابى بنظام التتر والسلبى تسجل نتيجته
If -ve so result have titre 1/40.
If +ve (ppt occur) so the titre 1/80 and repeat by dilution the serum with saline by ratio 1 : 1. Put 10 µ of serum mix 1 with 20 µ of reagents that gives +ve result only by the same steps.
If -ve so result 1/80
If +ve so the titre 1/160 and repeat by the same steps of dilution.
The titre of this test is :
1/40 , 1/80 , 1/160 , 1/320 , 1/640
The diagnostic titre is 1/160 .
ASOT
Anti Streptolysin-O-Titre
- نوع من انواع البكتريا من فصيلة streptococcus يصيب اللوزتين .
- تسبب صديد على اللوزتين وزيادتها تصل الى القلب وتسبب مشكله فى عضلة القلب .
***الاعراض :
-- المريض يكون اكثر عرضة للانفلوانزا بالاضافة الى الم فى المفاصل وخاصة الركبة والظهر وعدم القدرة على السير لمسافات طويلة وهذا يحدث غالبا عند الاطفال والشباب .
Procedure :
-->Reagent must have the room temp. before use then mix gently .
1.Put 10 µ of serum in a black slide then put on it 10 µ of the reagent .
2.Mix and rotate the slide by hands in a circle direction for 2 min .
-If precipitation occurs the result + ve. and give titre 200 IU / ml .
-If no ppt occurs the result is -ve .
-In +ve case we must repeat by the following steps:
1.Serum diluted by saline by the ratio 1 : 1 .
2.Take 10 µ from mix and put on the black slide then put 10 µ reagent mix and rotate for 2 min.
-If -ve so the titre 200 IU /ml .
-If +ve the titre 400 IU /ml . and repeat by the dilution of last mix by the ratio 1 : 1 or dilute the serum directly by The ratio 1 serum : 2 saline .and so on till the result give -ve .
The Titre in this test 200 - 400 - 600 - 800 IU/ml and no higher than 800 IU/ml .
IMPORTANT COMMENT:
-->False +ve result may occur in early infection and children between 6 months to 2 years .
RF
Rheumatoid Factor
هذا التحليل يكشف عن مرض الروماتيزم وذا المرض يوصف بانه auto immune disease
Procedure :
(As CRP steps) take 10 µ serum with 10 µ reagent of RF on black slide then rotate by hands for 3 min.
if -ve the result -ve .
if +ve the titre 8 mg/dl and repeat by dilution of serum with saline by ratio 1 : 1 [mix1].
10 µ mix1 with 10 µ reagent rotate for 3 min.
if -ve the result 8 mg/dl.
if +ve the titre 16 mg/dl and repeat by dilution of mix 1 by saline by ratio 1 : 1 and so on.
the titre of this test 8 , 16 , 32 , 64 , 128 , 256 .
IMPORTANT COMMENT
The test can be +ve with persons suffering from syphilis OR hepatitis C
CRP
C-Reactive Protein
هذا التحليل يكشف عن الالتهابات فى الدم .
Procedure:
-10µ serum + 10 µ reagent of CRF (على الشريحة السوداء) then mix with rotation by hands for 3 min.
If -ve so the result is -ve in report
If +ve the titre is 6 mg/dl and we will repeat by the following values:
-The serum diluted by saline by the ratio 1 : 1 [mix1] .
Then take 10 µ of mix with 10 µ of reagent and rotate for 3 min.
If -ve so the result 6 mg/dl .
If +ve so the titre 12 mg/dl and repeat by the following values :
-The mix will diluted by saline by the ratio 1 : 1 or the serum diluted by saline by ratio
1 serum : 2 saline [mix2].
Then take 10 µ of mix2 with 10 µ reagent and rotate for 3 min.
If -ve so the result 12 mg/dl .
If +ve so the titre 24 mg/dl and repeat by the same steps.
-Titre of this test : 6 , 12 , 24 , 48
If the result leads to more than 48 the report is more than 48 and not higher titre values .
IMPORTANT COMMENT :
When testing new born children false -ve result are possible .
تعليق