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Ham’s (acid hemolysin) test

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  • Ham’s (acid hemolysin) test

    Ham’s (acid hemolysin) test
    Definition:

    The Ham's (acid hemolysin) test looks for increased fragility of red blood cells in mild acid.


    Alternative Names:
    Ham test

    How the test is performed:

    Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and an elastic band or blood pressure cuff is placed around the upper arm to restrict blood flow through the vein. This causes vein to swell with blood.

    A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

    In infants or young children:

    The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.


    How to prepare for the test:

    There is no special preparation needed for this test.


    How the test will feel:

    When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.


    Why the test is performed:

    This test is used to confirm the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH).

    Diagnosis of PNH can be confirmed by having a positive acidified serum test (Ham test). In acidified serum, complement is activated by the alternate pathway. It binds to red blood cells, and ruptures the abnormal PNH cells, which are unusually susceptible to complement. With newer methods of diagnosis, such as flow cytometry, this test has become less important in the diagnosis of PNH.

    The Ham test is also positive in another rare disorder called congenital dyserythropoietic anemia, but in this case the sugar-water fragility test is negative. Furthermore, the clinical aspects of this disorder are not similar to PNH.

  • #2
    very good
    ولا تحسبن المجد تمرا انت اكله

    لن تبلغ المجد حتى تلعق الصبر

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    • #3
      thank u

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      • #4
        الف شكر لك على هذه المعلومات

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        • #5
          شكرااااااااااااااااااااااااااااااااااااااااا

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          • #6
            Thank you Mr.Tariq, PNH is an acquired stem cell disorder, in which all cells are affected, but with special stress on RBCs, but WBC, Platelets and RBCs are affected , this is why we can say that this disease can be characterized by pancytopenia (Low WBCs, RBCs, and Platelets). Also PNH can end as aplastic anemia with aplastic marrow., The problem that cells lack a factor that originally inhibits C3, but with the lack of DAF (decay acclerating factor) the cells will be abnormally susceptible to lysis by complement. The name nocturnal came from the fact the pH is lowered during sleep this will acclerate destruction of RBCs and manifested as hemoglobinuria. Thne another disease in which Mr. Tariq refered to is calle Congenital dyserythropoietic anemia (CDA) or also called HEMPAS( hereditary erythrocytic mulinuclearity with positive acidified serum test)
            thank you all
            من طلب العلا سهر الليالي

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            • #7
              thanxs for adding

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              • #8
                :sm170::sm170::sm170:الف شكر لك اخوووووووووووووووووووووووووووووي
                *- الإرادة ... والأمل .. قوتان إذا فلحت فى صهرهما ..... لصنعت المستحيل ... !!!

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                • #9
                  المشاركة الأصلية بواسطة عاشقة الميكرو26 مشاهدة المشاركة
                  :sm170::sm170::sm170:الف شكر لك اخوووووووووووووووووووووووووووووي
                  شكرا لتواجدك

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                  • #10
                    اين طريقة العمل والمواد المستخدمه لهامس تيست

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                    • #11
                      المشاركة الأصلية بواسطة حمود البدري مشاهدة المشاركة
                      اين طريقة العمل والمواد المستخدمه لهامس تيست
                      شكرا لتواجدك الموضوع كان تعرف للاختبار

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                      • #12
                        ham test كنا نسمية هم تيست لصعوبتة اخرة مرة عملتة كان من 12سنة أختبار قديم ومن النادر طلبة
                        :extra77:

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                        • #13
                          المشاركة الأصلية بواسطة dr.moz مشاهدة المشاركة
                          ham test كنا نسمية هم تيست لصعوبتة اخرة مرة عملتة كان من 12سنة أختبار قديم ومن النادر طلبة
                          نورت الصفحه و الموقع و الشبكه يا دكتور شكرا شكرا لمرورك

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