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حهاز الفلوسايتومتري (flow cytometry )

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  • حهاز الفلوسايتومتري (flow cytometry )

    اتمنى احد يفيدنا عن هذا الجهاز
    الي اعرفه عنه انه خاص بعينات البول ويقرأ مشرات ارتفاع
    كريات الدم البيضاء والحمراء وكذالك النيتريت وغيرها
    وهو عباره عن ثلاث اجزاء الجزء الاول من الجهاز لعينات اليورن
    بعدها خروجها من الجهاز الاول تدخل بالثاني والثالث مثل شاشه الحاسب يقرا ويعطي مؤشرات كل عينه
    فااتمنى من لديه خبره او معلومه عن هذا الجهاز يقدمها هنا
    وله من منا خالص الشكر والامتنان
    التعديل الأخير تم بواسطة عونى يوسف; الساعة 27-04-2010, 09:40 PM.

  • #2
    هل من مجيب؟ : (
    اتمنى ان اكون كتبت الاسم الصحيح للجهاز
    اعتقد هذا الاسم الصحيح له فلو سيتو متري لعينات اليورن فقط اتمنى الاجابه
    واعتقد انه تقنيه جديده على الاقل لدينا

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    • #3
      flow cytometer

      flow cytometer :its an immensely powerful tool for defining and enumerating lymphocytes. and its equpped to separate the identified cells is called fluorescent activated cell sorter (FACS


      )

      these instrument are used to study the properties of individual cells.
      monoclonal antibodies that bind to cell surface proteins expressed by different cell substrate labeled with fluorescent dyes and used to tag individual cells within a mixed population of cells.the mixture of cells is then forced through a nozzle to create a fine stream of laser beam; if the droplet contains a cell ,the cell will scatter the lazer light, while any dye molecules bound to the cell will be excited and fluoresce .sensitive photomultiplier tubes detect both the scattered light ,which gives information on the size and granulity of the cell, and the antibodies and hence on the expression of the cell surface proteins by each cell
      .
      [LEFT]when the cells are labeled with a single fluorescent antibodies , the data are usually displayed in the form of histogram of fluorescence intensity versus cell numbers. if two or more antibodies are used each coupled to different fluorescent dyes, then the data are more usually displayed in the form of two dimensional scatter diagram or as a contour digram, where the fluorescent of one dye -labeled antibodies is plotted against that of a second with the result that apopulation of cells labelling with one antibody can be further subdivided by its labelling with the second antibody. by examing large numbers of cells . the FACS can give quantitative data on the characterizes Bcells bearing different cell receptor associated molecules known as CD3,and the CD4and CD8 coreceptor protiens that distiguish the major T cell subset . the number of cells bearing distinct combination of these molecules and amount of each molecul expressed by each cell can be determine . finally the FACS can be used to identify cellswith selected charachteristic and then to separate these cells from all the other cells in the population .FACS analysis has beenn applied to abroad range of problems in immunology , such as the analysis of developing T cells in the thymus. indeed , the FACSplayed a vital role in the early identification of the AIDS as disease in which the CD4+ T cells are selectively depleted . flow cytometer offers one of the most versatile tools to study numerous functional and strucural aspects of the cells[/LEFT]



      أتمنى ان اكون قد اجبتك..
      .

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      • #4
        تفضل بالضغط على هذا الرابط وتتبع الشرائح

        هنا


        http://www.facebook.com/MkhtbrAlshjayt?ref=hl

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        • #5
          ستافتي
          الله يجعله بميزان حسناتك
          بس لو انه بالعربي لو ع الاقل الفكره الاساسيه للجهاز لكن ماقصرتي على كل الاحوال بحاول افهم الفكره الاساسيه منه
          الاستاذ عوني يوسف
          جزاك الله خيررر


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