Basic Principle of Electrophoresis
Principal of electrophoresis
Electrophoresis is the process of moving charged molecule in solution by applying an electric field across the mixture.
Principle of Separation
(1) According to charge
When charged molecules are placed in an electric field, they migrate toward either the positive (anode) or negative (cathode) pole according to their charge.
(2) According to size
1-Small
2-Large
Mice run faster through the forest than elephant
Instruments and Reagents
1. Buffer
2. Power supply
3. Supporting media
4. Detection and Quantification
I. Buffer
Function of buffer
1. carries the applied current
2. established the pH
3. determine the electric charge on the solute
High ionic strength of buffer
produce sharper band
produce more heat
Principal of electrophoresis
Electrophoresis is the process of moving charged molecule in solution by applying an electric field across the mixture.
Principle of Separation
(1) According to charge
When charged molecules are placed in an electric field, they migrate toward either the positive (anode) or negative (cathode) pole according to their charge.
(2) According to size
1-Small
2-Large
Mice run faster through the forest than elephant
Instruments and Reagents
1. Buffer
2. Power supply
3. Supporting media
4. Detection and Quantification
I. Buffer
Function of buffer
1. carries the applied current
2. established the pH
3. determine the electric charge on the solute
High ionic strength of buffer
produce sharper band
produce more heat
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