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سؤال: آلية الكحول 5% في كبح اجتياح البروتوس في وسط الاغار الدموي

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  • سؤال: آلية الكحول 5% في كبح اجتياح البروتوس في وسط الاغار الدموي

    السلام عليكم اعضاء المنتدى الكرام


    عندي سؤال ورد في الامتحان العملي واتمنى الاجابة عليه لمن يمتلك معلومات او يخدمنا في اعطاء رابط لموقع للاحياء الدقيقة وجزاكم الله كل الخير

    السؤال هو : ما آلية الكحول 5% في كبح اجتياح البروتوس في وسط الاغار الدموي او اي وسط آخر , وماهي المركبات الاخرى التي تثبط الاجتياح ؟

  • #2
    و عليكم السلام أخي الكريم ..

    فهمت سؤالك كما يلي : كيف ممكن أمنع أو inhibite الــ swarming لــ proteuos على الــ bloog agar ??

    أعتقد ان الجواب و الله أعلم :

    دور الكحول هنا كما يلي:

    Reduced surface moisture

    As swarming is dependent upon the surface moisture, use of dried agar plates or agar plates treated with alcohol prior to inoculation have been recommended to prevent this phenomenon. However this method is not very reliable as the dryness required to inhibit swarming, would completely suppress the growth of fastidious organisms


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    وماهي المركبات الاخرى التي تثبط الاجتياح ؟

    هناك أكثر من طريقة


    Growth inhibitors

    Several antibiotics such as neomycin, sulfonamides, combination of polymyxin and inhibitors such as chloral hydrate and sodium azide were also found to suppress swarming. The relative efficiency of several metabolic inhibitors had been determined in terms of growth-inhibiting concentration (GIC)/swarm cell-inhibiting concentration (SIC) ratio. GIC/SIC ratio was reported to be 15.0 for streptomycin and 27.0 for proflavin



    Inhibition of flagellar function

    Several compounds were reported to inhibit swarming by disturbing the structural integrity or activity of flagella. For instance, addition of ferrous salt to proteose peptone medium inhibited the swarming of Proteus without affecting its growth, and Proteus cells grown in an equivalent liquid medium were nonmotile. Similarly, boric acid inhibited the swarming on blood agar medium without affecting the growth of Neisseria gonorrhoeae. Boric acid was shown to form a complex with the flagellar protein that caused disintegration of flagella. Sodium alkyl sulphate (a detergent) was also reported to be an effective antiswarming agent and its efficiency increased with increasing chain length (GIC/SIC = 10). At the same time, ßphenethyl alcohol (GIC/SIC = 5.0) and EDTA at a concentration of 2 mM or higher was also found to inhibit flagellation and thereby prevent swarming.

    Conclusion:
    Ferrous salt to proteose peptone medium
    Boric acid
    EDTA at a concentration of 2mM



    Inhibition by unique mechanism

    Inhibition by some yet unexplained mechanisms has also been reported. In this category, the use of electrolyte deficient media for inhibition of swarming has been recommended. Activated charcoal at a concentration of 0.5% w/v or higher was also reported to inhibit swarming. Although PNPG (p-nitrophenyl glycerol) did not affect flagellation or motility, it could inhibit differentiation, thereby acting as an
    effective anti-swarm agent.



    أتمنى إن الإجابات تكون صحيحة ... و لو عرفت انت ال‘جابات ياليت تشاركنا فيها حتى نستفيد كلنا ..


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    • #3
      شكرا جزيلا يا (الواقع والحياد ) على الاجابة وآسف على تأخري بالرد بسبب مشاكل الشبكة عندنا في سوريا , واكيد سوف اطلعكم على الاجابة عندما احصل عليها , وانا حاليا أحضر للامتحان العملي وسوف نستعين بكم في الاجابة على بعض الاسئلة التي لم اجدهافي مقرراتنا , وآمل ان تجيبوني عليها وبارك الله بكم والسلام

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