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سؤال عن Neisseria & Heamophilus

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  • سؤال عن Neisseria & Heamophilus

    how do we differentiate between Neisseria and Haemophilus other than that Neisseria is a diplococcus and heamophilus is a coccobacillus?

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    A specimen with H. influenzae is Gram-stained to show Gram-negative, coccobacilli, with no specific arrangement. Then, it's grown on nutrient agar plate with added X & V factors. Also, growth on blood agar would show the satellitism phenomenon, which is the growth of H. influenzae around a streak of S. aureus line of growth. On Chocolate agar, the organism grows readily as this medium is nothing but a heated blood agar, which means that RBC's are lysed upon heating, releasing factor V for the organism to grow (X is already in blood). It shows small, convex, smooth, pale, grey or transparent colonies. The organism is also catalase and oxidase positive. Serological testing demonstrates the capsular polysaccharide, which is usually of the b type as it's the most common to cause infections.


    المصدر ===> http://en.wikipedia.org/wiki/Haemophilus_influenzae

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