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How to culture stool?

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  • How to culture stool?

    Hi everybody

    Actually I am on job training n am wondering about different matters

    especially in Bacteriology lab


    SO


    Am asking u to mention the different types of media that we should culture stool on

    and what is the different tests that we should do it in the 2nd day & 3rd day?


    :sm180:

  • #2
    In the frist day culture the stool in the fallowing media :
    1/ XLD( xylose lysine deoxycolate): for salmonella and shegilla
    2/ macconkey agar : for coliform
    3/TCBS: for vibro cholera
    4/ campy : for campylobacter
    In frist day direct and indirect inoculation
    Direct : in all above media
    Indirect : culture in selenite F broth( for enrichment the salmonella and shegilla) and alkaline peptone water ( for enrichment the vibro )and then put in incubator
    In second day culture from enrichment broth in the above media
    from selenite F broth in XLD and macconkey agar and from alkaline peptone water in TCBS
    and put in incubater .
    in third day see this media and interpretation the result

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    • #3
      هذي الــ manulal اطبعيها و خليها معاك يا نحوسه و ركزي على الكلتشر من صفحه 3 ...

      http://microbiology.mtsinai.on.ca/manual/ent/ent01.pdf




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