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  • طلب توضيح لقراءة الـresult..

    السلام عليكم ورحمة الله...


    في موضوع urin culture و ear smear culuter ..ممكن توضيح الطريقه الصحيحه لعمل هذه المزرعه..وكيفية قراءة الـresult..

  • #2
    شكرا على هالسؤال
    اتمنى من الاخوان مساعدتنا بالتفاصيل وخاصة colony count
    ]



    'Life is too

    short, so forgive quickly. Believe slowly. Love truly.

    Laugh uncontrollably. Never regret anything that makes

    you happy. And have a wonderful journey!!!'

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    • #3
      this is 4 Urine culture


      Inoculation
      The specimen is typically inoculated onto two plates: 1) blood agar and; 2) a gram-negative
      plate (EMB, MAC etc.), since most UTIs are caused by gram-negative bacteria.

      As previously
      mentioned, both the types of bacteria as well as the number of bacteria present in the specimen are
      important. Therefore, some method of quantifying the bacteria is used.

      Most often a calibrated
      loop is used. Inoculating loops (platinum or disposable), calibrated to 0.01 ml or 0.001 ml of fluid
      are immersed in the urine sample and streaked onto the media.

      After incubation, the number of
      bacteria in the specimen can be estimated by counting the number of colonies that grow.

      The number
      of colonies on the media divided by the volume of the calibrated loop gives the concentration of
      bacteria per ml of the original specimen.

      Microbiological Diagnosis of UTI
      Criteria for workup (identification and antimicrobial sensitivity testing) depend on two
      factors: 1) the number of bacteria present and ; 2) the kinds of bacteria present.

      As mentioned
      previously, urine becomes contaminated when passing from the bladder over the urethral membranes.
      The finding of such normal flora in a culture would therefore not necessarily indicate infection.

      Since
      a catheter specimen or a suprapubic aspirate bypasses this route, any growth is considered significant.


      Therefore, the rest of this discussion will concern the properly collected CCMS results.
      Significant bacteriuria occurs when there are 100,000 colony forming units per ml in a
      properly collected CCMS urine.

      Specimens containing between 10,000 and 100,000 colonies of a
      single microbial species per ml represent possible or probable infection and should be repeated,
      whereas those with less than 10,000 colonies per ml represent contamination.

      How to culture???i

      The urine specimen is mixed and a sterile calibrated loop is dipped vertically into the sample. The loop is streaked down the centre of the plate and then cross-streaked at a 90 angle to the inoculum
      .
      ان عشت فعش حرا أومت كالاشجار وقوفا


      sigpic

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      • #4
        or u can use this web for more details in urine culture

        http://microbiology.mtsinai.on.ca/ma...rine/mi_ur.pdf
        ان عشت فعش حرا أومت كالاشجار وقوفا


        sigpic

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        • #5
          المشاركة الأصلية بواسطة soma مشاهدة المشاركة
          this is 4 Urine culture


          Inoculation
          The specimen is typically inoculated onto two plates: 1) blood agar and; 2) a gram-negative
          plate (EMB, MAC etc.), since most UTIs are caused by gram-negative bacteria.

          As previously
          mentioned, both the types of bacteria as well as the number of bacteria present in the specimen are
          important. Therefore, some method of quantifying the bacteria is used.

          Most often a calibrated
          loop is used. Inoculating loops (platinum or disposable), calibrated to 0.01 ml or 0.001 ml of fluid
          are immersed in the urine sample and streaked onto the media.

          After incubation, the number of
          bacteria in the specimen can be estimated by counting the number of colonies that grow.

          The number
          of colonies on the media divided by the volume of the calibrated loop gives the concentration of
          bacteria per ml of the original specimen.

          Microbiological Diagnosis of UTI
          Criteria for workup (identification and antimicrobial sensitivity testing) depend on two
          factors: 1) the number of bacteria present and ; 2) the kinds of bacteria present.

          As mentioned
          previously, urine becomes contaminated when passing from the bladder over the urethral membranes.
          The finding of such normal flora in a culture would therefore not necessarily indicate infection.

          Since
          a catheter specimen or a suprapubic aspirate bypasses this route, any growth is considered significant.


          Therefore, the rest of this discussion will concern the properly collected CCMS results.
          Significant bacteriuria occurs when there are 100,000 colony forming units per ml in a
          properly collected CCMS urine.

          Specimens containing between 10,000 and 100,000 colonies of a
          single microbial species per ml represent possible or probable infection and should be repeated,
          whereas those with less than 10,000 colonies per ml represent contamination.

          How to culture???i

          The urine specimen is mixed and a sterile calibrated loop is dipped vertically into the sample. The loop is streaked down the centre of the plate and then cross-streaked at a 90 angle to the inoculum
          .
          http://people.uis.edu/jvese1/BIO347L...neCultures.pdf
          http://www5.0zz0.com/2012/03/18/21/288835025.jpg

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          • #6
            regarding proccessing its as our colleague vsaid but regarding reporting you have to consider many factors
            1.consisider nomber of colonies if more than 100000cfu
            2.nomber and type of colonies eg if more than three types consider contamination
            3. if less than 3 but diphteroid,lactobacilli consider contamination of skin flora even if significant nomber
            if patient from icu or high risk area and cause of fever not known then even if more than 3types eg diphteroid,lactobacillus and other 2 pathogen like eg E.coli and strept groupB then you have to identify the 2 pathogen
            5 if pt with sevier symptoms then you have to identify pure growth even with law count eg 100cfu
            6. if pt on antibiotic you have to identify any low count if equal or less than 3types
            7. if pt with recurrent uti then you have to identify any low count
            8.if pt immuonocompromised egif pt withAIDS or on chemotherapy then you have to identify even normal flora if pure growth and pt is symptomatic

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            • #7
              يعطيكم الف الف الف عافيه عالتوضيح..مرسي كتييييير وماقصرتوا..ربي يجعلوا في موازين اعمالكم..

              شكـــــــــــرا جزيلا..

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              • #8
                السلام عليكم و رحمة الله و بركاته

                اول بالنسبة لعمل urine culture

                قبل اى شئ لازم نتاكد ان المريض متوقف عن اخذ اى مضاد حيوى لمده 48 ساعة على الاقل قبل اخذ عينه للمزرعه.

                1- نعطى المريض كوب معقم مخصوص لهذا الغرض وهى متوفرة فى اى مختبر.


                2-نطلب من لمريض احضار عينة من منتصف البول لا من اوله ولا من اخر البول.

                3-التاكيد على المريض بالحفاظ على الكوب مغلق ولا يفتح الا عند وضع العينه به ثم يغلق مباشرة فور الانتهاء من تجميع العينه لتجنب ال contamination

                4- نقوم بعمل فيلم مباشر من العينه دون صبغات للتعرف على نسبة اال pus cells بالعينه و عددها فى H.P.F وممكن اعمل nitrite test و ده بيدينى indication لو عندى gram -ve

                5- نقوم بزرع العينة على "CLED , mackonky, and blood agar" و غالبا ممكن نكتفى بال blood و ال Mackonky agar باستخدام swap or loop بان نقوم بغمس ال swap فى العينه ثم زرعها فى الطبق على شكل زجزاج او اى طريقه اخرى بس انا بفضل الزجزاج لانه يترك اكبر مساحه فارغه بالطبق ممكن استخدمها control علشان لو فى contamination اقدر اتعرف كويس على الميكروب المعزول من العينه من ال contaminated

                6- نقوم بوضع الطبق بالحضانه عند 35 - 37 درجه مئوية لمده من 12 - 48 ساعة المهم انى احصل على growth كويس اقدر اشتغل عليه بعد كده

                7- نقوم بعمل gram stain ومنها ممكن نحدد الميكروب اللى انا عزلته هل هو gram -ve or gram +ve و بشوف شكل الميكروب هل هو cocci or bacilli و ممكن نكتفى لحد هنا فى تعريف الميكروب و لو عايزين نكمل تعريف اكتر يبقى كده الموضوع هيكبر مننا

                8- sensitivity test و هنا هنختار طبق يكون اكبر شويه علشان نزرع عليه اكبر عدد من ال antibiotics و المره دى انا باخد الميكروب اللى انا كبرته و اخد منه بال swap و افرده فى الطبق الكبير و ااكد ان الميكروب اتفرد فى كل الطبق و مفيش مكان فاضى بان انا بعد ما افرد اغير زاوية الطبق بانى الفه 45 درجة و افرد تانى و اغير 45 درجة و افرد تانى و 45 درجة و افرد تانى بكده يبقى انا متاكد انى فردت الميكروب فى كل الطبق صح و ازرع بعد كده ال antibiotics بتاعتى و اراعى انا اسيب مسافه بين كل antibiotic و التانى علشان اقدر اقرا النتيجه صح و على حسب حجم ال zone of inhibition بحدد ال sensitivity

                9- الميكروب اما انه يكون (susseptible او intermediate susceptibility او resistant )

                10- دى بعض لينكات للى عايز معلومات اكتر

                هنا ممكن نعرف ايه هى البكتريا اللى ممكن تقابلنا فى عينات ال urine
                http://www.medicinenet.com/urine_infection/article.htm

                وهنا ازاى نعمل gram stain
                http://www.rlc.dcccd.edu/mathsci/rey...nual/gram.html

                وهنا ازاى نقرا نتيجة ال sensitivity test
                http://www.rlc.dcccd.edu/mathsci/rey...tibiotics.html

                و يا رب اكون قدرت افيدكم بمعلوماتى المتواضعه و لو فى اى استفسار انا موجود تحت امركم

                " حينمــا يفتخــر الناس بحســن كلامهــم

                ,,,,,,,, افتخــر أنا بحســن الصــمت " .

                MR HIDE

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                • #9
                  EAR SPECIMENS - EAR SWABS

                  http://microbiology.mtsinai.on.ca/ma...d/wnd13_01.pdf

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                  • #10
                    شكرا اختى الواقع و الحياد على اكمال الموضوع و فعلا الملف ده موضح كل حاجه عن ال ear swap

                    " حينمــا يفتخــر الناس بحســن كلامهــم

                    ,,,,,,,, افتخــر أنا بحســن الصــمت " .

                    MR HIDE

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