how can you trat the section??

[kind rose]

بليييييييييييييييز ساعدوني

how can you trat the section to remove formaline pigmentation from tissue?

how can you treat the section to remove the melanin pigmentation from tissue??


list the special staining techniques and reagent that used to demonestrate the following pigments
1- iron in heamosidren
2- melanin
3- urate
4- bilirubin
5- calcium
6- cubber
7- lipofushion



وشكرا

[white falcon]

http://www.hoslink.com/histo/8.htm

:)

[ريووووف]

شوفي ذا الرابط وان شاء الله يفيدكـ,,,,,
http://www.histology-world.com/stains/stains.htm

[asma_10]

ان شاء الله سوف اجهز لك الحاجات التي طلبتها

[Son-Son]

ابشري لقيت عنها كثيييييير.........


How can treat the section to remove formalin pigmentationfrom tissue?
It is easily removed with alcohol - ammonia solution (ALCOHOL-AMMONIA SOLUTION 95 % alcohol 50 mL Concentrated ammonia (0.880) 15 mL Mix before use)
PROCEDURE:
1. Deparaffinize and hydrate to distilled water.
2. Place in Alcohol-ammonia solution for 1 (one) hour.
3. Wash in water.
4. Stain using H&E or other technique.
How can treat the section to remove melanin pigmentation from tissue?
Melanin can be bleached by strong oxidising agents such as potassium permanganate
SPECIMEN:
Standard paraffin sections, formalin fixed
CONTROL :
H&E without bleaching procedure, and H&E with bleaching procedure for each patient.
REAGENTS:
(A) 0.25 % AQUEOUS POTASSIUM PERMANGANATE
(B) 5 % AQUEOUS OXALIC ACID
PROCEDURE:
1. Deparaffinize and hydrate to distilled water.
2. Oxidise in permanganate for 30 minutes.
3. Water wash.
4. Bleach in oxalic acid until white.
5. Proceed staining as desired.
RESULTS:
Skin melanin is bleached within 30 minutes Ocular melanin takes 2 - 4 hours.





List the special staining technique used to demonstrate the following pigments?
Pigment technique reagent
Iron in hemosisidrin PERL'S PRUSSIAN BLUE FOR FERRIC IRON (A) 2 % AQUEOUS POTASSIUM FERROCYANIDE
(B) 2 % AQUEOUS HYDROCHLORIC ACID
( C) 1 % AQUEOUS NEUTRAL RED
Melanin MASSON - FONTANA METHOD (A) PREPARATION OF SILVER SOLUTION To 20 mL of 10 % silver nitrate add concentrated ammonia drop by drop until the formed precipitate almost dissolves. A faint opalescence is evident when the end point is reached. If too much ammonia is added, 10% silver nitrate can be added drop by drop until the faint opalescence is obtained. To this silver solution add 20 mL of distilled water and filter. Store in a dark bottle. The solution will last for about four weeks in fridge 4�C.
(B) 5 % SODIUM THIOSULPHATE
(C) 1 % NEUTRAL RED

Urate Staining with eosin only (no haematoxylin) provides limited contact with water Eosin as used in linear stainer for routine H&E
Billurubin VAN GIESON METHOD
(A) FOUCHETS REAGENT 25 % trichloroacetic acid (aq) 25 mL 10 % ferric chloride (aq) [corrosive] 25 mL
(B) VAN GIESON'S SOLUTION (see Van Gieson's method 2.4) or SIRIUS RED STAIN
Calcium MODIFIED VON KOSSA'S METHOD (A) 1 % AQUEOUS SILVER NITRATE
(B) 2.5 % SODIUM THIOSULPHATE
(C) 1 % NEUTRAL RED OR VAN GIESON'S PICRO-FUCHSIN
Copper TIMM'S COPPER STAIN (A) SOLUTION A 5.0 % Silver nitrate aqueous solution.
(B) SOLUTION B Hydroquinone 2.0 g Citric acid 5.0 g Distilled water 100.0 mL This solution can be stored at 4�C in the dark for one month.
(C) 0.1N HCl or 15% TRICHLORACETIC ACID
lipofuscin Sudan black B
Periodic-acid-schiff
Red oil 0.5% Periodic Acid:
Periodic acid 0.5 gm
Distilled water 100.0 ml

[kind rose]

شكرا لكي ألأختي ريوف

[kind rose]

شكرا لكي أختي سن سن
:-)

[dr\daiana]

شكرا لكم جميعا
صراحه افدتوني ووفرتوا عليا الكثير

مشكورررررررررررين