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myco bacterium

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  • myco bacterium

    Georgia: mycobacterimI
    meanse fungus like bacleria, growth on broth media like mold.

    diffinition:
    obligate aerobe like l,j media growth in slope

    slender ,slightly eurved rods "weakly" not stained ewith grams.

    non motile non sporing non capsulated

    Myco Bacterum tuber elusis oblgat erobe acid fast bacilli growin
    artifical media
    AFB:means dont stained by gramstain but by zn stain

    disease: tuber clousis
    transimission:
    by in halition of dropletes
    person to person transimisson
    pathogenicity: plumonary tuber culosis
    in halation of infected droplet
    التعديل الأخير تم بواسطة روبي; الساعة 02-04-2008, 01:06 AM.
    ...روبي...

  • #2
    Thanks I have Q here about mcobacterium tuberclusis What the Lab diagnonosis if i cause UTI ?

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    • #3
      SIZE="4"][/SIZE]Hello every one . I just would like to anwer the question that been asked by our brother
      Abubakar Ahmed

      WHO ASKED ABOUT THE LAB. DIAGNOSIS OF TB

      So, to begin

      Firstly :

      When we talk about TB DIAGNOSIS we should know

      Which Samples??
      Sputum

      Blood

      Cerebrospinal fluid (CSF)

      A biopsy of infected lung tissue

      Some advice
      Collecting more than one specimen per patient is better.
      Morning sample is the most suitable
      Sample should be collected in a sterile container



      And

      Type of the room and the safety cabinet

      TB samples should be processed inside of safety cabinet class 3
      Which is a close system conducted through attached rapper gloves and the air inside is filtered. This cabinet inside a room with negative pressure.





      Secondly

      Lab diagnosis usually will include:

      Direct examination


      Staining with Ziehl Neelsen method

      The International Standard format :

      Few <10 cells/film

      1+ 10-20 cells/film

      2+ 20-30 cells/film

      3+ >30 cells/film



      Culturing of the clinical samples on :

      A simultaneous Lowenstein-Jensen LJ slope is inoculated

      Blood samples are inoculated into Mycobacterial growth indicator tube MGIT

      Using Bactec system

      Routine method used in culturing blood samples
      Culture tubes contain a fluorescent sensor at the bottom which responds to the concentration of oxygen .
      Initial concentration of dissolved oxygen quenches the emission from the compound, and little fluorescence can be detected.
      Actively respiring microorganisms consume the oxygen which allows the compound to fluorescence.




      Finally

      Identification of Acid Fast Bacilli (AFB)

      Growth at 37oC

      But not at 25oC or 45oC

      Aerobic

      No growth in presence of thiacetazone

      Non pigmented neither in the dark nor in the light

      It may or may not hydrolyse the tween


      In Histopathology Lab. The bacteria can be detected within the tissue of the taken biopsy


      Molecular testing can be used as a tool to diagnose this bacteria. However, this facility may not be available in all laboratories

      Thank you all guys and girls

      your Sincerely,

      Medical and Molecular. [/SIZE]
      Address people in the language they can understand
      خاطب الناس على قدر عقولهم:sm188:

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      • #4
        الله يعطيك العافيه على هذه المعلومات عن جد رااااااائع , الى اللقاء

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        • #5
          الله يعافيك Hematolgist وشكرا على المرور وربي ينفع الجميع , الى اللقاء عزيزي
          Address people in the language they can understand
          خاطب الناس على قدر عقولهم:sm188:

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          • #6
            مرحبا بكم...أنشالله تكونو أستفدتو(أنا بنت...روبي)
            شكرا لكل من قرأ هذا البوست....
            ...روبي...

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            • #7
              مشكوووووووووووووور أخي الغالي
              الحياة مليئة بالحجارة..فلا تتعثر بها بل اجمعها وابن بها سّلما تصعد به نحو النجاح

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              • #8
                تسلمى دكتورة روبى على المعلومات القيمة التى طرحتيها
                مهما طال الليل لابد من صباح :sm200::sm188::sm199::sm187::sm198:

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                • #9
                  أشكر Medical and Molecular و روبي على مناقشة هذا المرض الخطير

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                  • #10
                    thax alot, you r very helpful

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                    • #11
                      مشكوووووووووووووور أخي العزيز ع المناقش الممتعة

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