ما أهمية استخدام oil immersion objective ? بالأصح ما أهمية ال oil ؟
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oil immersion objective
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Because the Light passing out of the slide, into the air, toward the objective lens is refracted, due to the different in refractive index between air and glass. While the bending cause by this difference is not important at 100X and 400X, at 1000X this refraction is problematic, causing blurring of the image and significant loss of light. Immersion oil has a refractive index very similar to that of glass. Placement of a drop of oil between the objective lens and the slide prevents the bending of light rays and clarifies the image. The blue dashed line represents a potential light ray if immersion oil is not present. The red dashed line represents a light ray if immersion oil is present.
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