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مشاهدة النسخة كاملة : ماهي الصبغات المستخدمه لل carbohydrate

سموووووي
15-05-2008, 03:33 PM
ماهي الصبغات المستخدمه لل carbohydrate

ياليت تجاوبوني لو سمحتو؟
juju86qayyas
16-05-2008, 11:06 AM
mr7ba:)
اهم صبغة للكربوهيدرات حسب معلوماتي
PAS(Periodic Acid Shiff's staining)
the glycogen after this stain is magenta red color

مابعرف اذا في شي اخر بس هاي الاهم:)
وانشالله يكون كلامي مزبوط:))
antivirus
16-05-2008, 11:56 AM
تفضلي الرابط هذا موجود فية Histology Procedure Manuals

http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

إن شاء الله تستفيدين منه
الواقع والحياد
16-05-2008, 12:16 PM
Periodic Acid-Schiff Method (PAS)


Principally used to demonstrate structures rich in carbohydrate macromolecules such as glycogen, glycoprotein, and proteoglycans found in ground substance of connective tissues, basement membranes and mucus. This method depends on the selective oxidation by periodic acid of free hydroxyl groups on two adjacent hydroxyl groups converting the alcohols to aldehydes. The aldehydes are then detected by the Schiff reagent, which stains them reddish purple. Other tissue components stain according to the counterstain used. Lead-hematoxylin or another basic stain is often the counterstain

http://www.arabslab.com/vb/uploaded/8_01210929205.jpg (http://www.arabslab.com/vb)
http://www.arabslab.com/vb/uploaded/8_01210929102.jpg (http://www.arabslab.com/vb)



PAS - McMANNUS' PERIODIC ACID SCHIFF'S ..... click here (http://www.arabslab.com/vb/uploaded/8_01210929330.zip)