This is a test involving special staining and direct microscopic examination of a sputum specimen, used to check for the presence of Mycobacteria.
How is the Test Performed?
Obtain a coughed sputum sample. You are asked to cough deeply and expel any material that comes from the lungs (sputum) into a container. If sputum is not produced, sometimes bronchoscopy will be performed.
The specimen is spread on a microscope slide, which is called a smear. The cells of the specimen are stained with a dye, carbol-fuchsin, heat and acid are applied, and then it is counterstained with methylene blue.
The prepared specimen is then examined under the microscope. If the stain shows mycobacteria, the specimen may be placed in culture media. (Specimens are often cultured even if no mycobacteria are seen, since sometimes the numbers are so low that it is hard to see them.)
How to Prepare for the Test?
It can help to drink a lot of fluids the night before the test. It enhances the accuracy of the test if done first thing in the morning.
How will the Test Feel?
There is no discomfort, unless a bronchoscopy needs to be performed.
Normal Values
The presence of no mycobacterial organisms isnormal.
What do Abnormal Results Mean?
Abnormal results show that the stain is positive for Mycobacterium tuberculosis, Mycobacterium avium-intracellular, or other Mycobacteria or acid-fast bacteria.
Special Considerations
To increase the accuracy of this test, it is sometimes done three times, often on three consecutive days.
There are more sophisticated tests that are sometimes used to stain sputum for mycobacteria. Check with your health care provider to see if these are available in the laboratory.
Risks
There are no risks, unless bronchoscopy is performed.
وفي الخـــتــــام اضع لكي في المرفقات ملف عن خطوات الصبغة خطوة بخطوة
* ملاحظة : اذا لم تكن الصور واضحة بامكانك ان تزيد تكبير الخط وسوف تصبح اوضح .
السلام عليكم
شكرا ويعطيك العافية ,,,,,,,,,,,,,,,,,,,,,بس جهازي ما قبل يفتح الملف المرفق .
ثانيا : عملية الزرع في L J M وطريقة التحضين ...ممكن تشرحها ؟
شكر و عذرا
السلام عليكم
شكرا ويعطيك العافية ,,,,,,,,,,,,,,,,,,,,,بس جهازي ما قبل يفتح الملف المرفق .
ثانيا : عملية الزرع في L J M وطريقة التحضين ...ممكن تشرحها ؟
شكر و عذرا
تعليق