الواقع والحياد
22-11-2006, 09:45 PM
Body Fluids
There are a number of fluids analyzed from the body that help the physician in diagnosis.
The following fluids are evaluated within the hematology lab for appearance, clarity, and the number of cells in it:
cerebrospinal fluid
pleural and pericardial fluid
peritoneal fluid
synovial fluid
The gross examination of the fluid is done by describing the color and character of the fluid. Each fluid has a normal color and character. The cell count is done on the counting chamber undiluted if possible. Electronic cell counters should not be used for cerebrospinal fluid. The fluids must be done promptly, otherwise cell lysis gives a false impression of the number of WBC and RBC's present. The fluids are examined microscopically and evaluated for any abnormal morphology of the cells. Diseases such as bacterial meningitis, cryptococcal meningitis, carcinoma, lymphoma, and many more can be diagnosed from the evaluation of these fluids.
Method for Obtaining Body Fluid Counts
The color and character of the body fluid is described and reported.
The body fluid is loaded on the hemacytometer undiluted
http://www.gomcl.com/hema/fluids/hemo.gif
and the number of red blood cells and white blood cells are counted in the entire ruled area (9 squares)
http://www.gomcl.com/hema/fluids/square.gif
If the body fluid is too cellular to be counted accurately, it must be diluted.
If the WBC is greater than 5/cubic mm, a slide is made and microscopically examined.
A Sample Slide
http://www.gomcl.com/hema/fluids/malig1.jpg
Wright Stain Smear of Malignant Cells in Spinal Fluid
There are a number of fluids analyzed from the body that help the physician in diagnosis.
The following fluids are evaluated within the hematology lab for appearance, clarity, and the number of cells in it:
cerebrospinal fluid
pleural and pericardial fluid
peritoneal fluid
synovial fluid
The gross examination of the fluid is done by describing the color and character of the fluid. Each fluid has a normal color and character. The cell count is done on the counting chamber undiluted if possible. Electronic cell counters should not be used for cerebrospinal fluid. The fluids must be done promptly, otherwise cell lysis gives a false impression of the number of WBC and RBC's present. The fluids are examined microscopically and evaluated for any abnormal morphology of the cells. Diseases such as bacterial meningitis, cryptococcal meningitis, carcinoma, lymphoma, and many more can be diagnosed from the evaluation of these fluids.
Method for Obtaining Body Fluid Counts
The color and character of the body fluid is described and reported.
The body fluid is loaded on the hemacytometer undiluted
http://www.gomcl.com/hema/fluids/hemo.gif
and the number of red blood cells and white blood cells are counted in the entire ruled area (9 squares)
http://www.gomcl.com/hema/fluids/square.gif
If the body fluid is too cellular to be counted accurately, it must be diluted.
If the WBC is greater than 5/cubic mm, a slide is made and microscopically examined.
A Sample Slide
http://www.gomcl.com/hema/fluids/malig1.jpg
Wright Stain Smear of Malignant Cells in Spinal Fluid