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قسم الطفيليات و كذلك الفطريات
http://www.atlas.arabslab.com/
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للإجابه على سؤالك الثاني :
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[align=left]The principle of the enzyme-linked immunosorbent assay (ELISA).
To detect antigen A, purified antibody specific for antigen A is linked chemically to an enzyme. The samples to be tested are coated onto the surface of plastic wells to which they bind nonspecifically; residual sticky sites on the plastic are blocked by adding irrelevant proteins (not shown). The labeled antibody is then added to the wells under conditions where nonspecific binding is prevented, so that only binding to antigen A causes the labeled antibody to be retained on the surface. Unbound labeled antibody is removed from all wells by washing, and bound antibody is detected by an enzyme-dependent color-change reaction. This assay allows arrays of wells known as microtiter plates to be read in fiberoptic multichannel spectrometers, greatly speeding the assay. Modifications of this basic assay allow antibody or antigen in unknown samples to be measured [/align]
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[align=left]The Spectrophotometer
The absorbance is measured by an instrument called a spectrophotometer. The essential parts of a spectrophotometer include a light source, a wavelength selection device such as a monochromator or filter, a sample chamber, a light detector, and a readout device, usually also include a computer, which is used for storage and analysis of the spectra. The most useful machines scan the wavelength of the light that is incident on the sample and produce, as output, spectra of absorbance versus wavelength.[/align]
[web]http://3e.plantphys.net/image.php?id=71[/web]
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[align=center][align=right]ماشاء الله ...
الواقع والحياد كانها ديكشنري
عيوني عليك بارده>>>>>>> لايقولوا حسود
والله يوفقك ياختي...
اتوقع اخي الدكتور الاخت جاوبت على اسئلتك......
[glow=00CC99]سلاااااام[/glow][/align][/align]
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