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PYR
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the PYR test is commonly performed to separate Enterococcus species (positive reaction) from the Group D streptococci (S. bovis, S. equinus), which are negative.It should be remembered that Streptococcus pyogenes (group A) also produces PYR; therefore, additional characteristics such as beta hemolysis are important.Some species of Aerococcus and Gemella are also PYR-positive; however, they can be suspected if large cocci in tetrads or clusters are observed on gram stain.These species are rare isolates in most clinical practices.
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المشاركة الأصلية بواسطة المتميزة مشاهدة المشاركةthe PYR test is commonly performed to separate Enterococcus species (positive reaction) from the Group D streptococci (S. bovis, S. equinus), which are negative.It should be remembered that Streptococcus pyogenes (group A) also produces PYR; therefore, additional characteristics such as beta hemolysis are important.Some species of Aerococcus and Gemella are also PYR-positive; however, they can be suspected if large cocci in tetrads or clusters are observed on gram stain.These species are rare isolates in most clinical practices.
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PYR REAGENT
Cat. #Z127 PYR Reagent 15ml
INTENDED USE
Hardy Diagnostics PYR Reagent is recommended for use in conjunction with PYR testing for the detection of pyroglutamate aminopeptidase found in group A streptococci (Streptococcus pyogenes) and group D enterococci. The PYR Reagent combines with beta-naphthyl amide released during pyroglutamate aminopeptidase (PYR) activity to form a pink to red pigment.
SUMMARY
PYR is a rapid colorimetric method for the presumptive identification of group A streptococci and enterococci based on pyroglutamate aminopeptidase activity. L-pyroglutamic acid beta-naphthylamide impregnated into the test disk serves as the substrate for the detection of pyroglutamate aminopeptidase. Hydrolysis of the substrate yields beta-naphthylamide which combines with the PYR Reagent to form a pink to cherry red pigment.
REAGENT FORMULA
The PYR Reagent contains 0.015% p-dimethylaminocinnamaldehyde per liter.
STORAGE AND ****F LIFE
Upon receipt store at 2-30 degrees C. Product should not be used if there are any signs of deterioration or if the expiration date has passed. Protect reagent from freezing.
The expiration date applies to the product in its intact packaging when stored as directed.
This product has the following ****f life from the date of manufacture:
365 Days: Z127 PYR Reagent
Refer to the keyword "Storage", in the Hardy Diagnostics software program HUGO, for more information on storing culture media.
PRECAUTIONS
For in vitro diagnostic use only. Observe approved biohazard precautions and aseptic techniques. To be used only by adequately trained and qualified laboratory personnel. Sterilize all biohazard waste before disposal. Do not ingest. Avoid skin contact.
Refer to the keyword "Precautions", in the Hardy Diagnostics software program HUGO, for more information regarding general precautions when using culture media.
PROCEDURE
Specimen Collection: This product is not intended for primary isolation of patient specimens. It should be used only with cultures of isolated organism. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.
Method of Use: The PYR Reagent is designed for use in the PYR Test Kit (Cat. #Z75), or with the Esculin/PYR Screen. Consult the appropriate technical insert for the use of this product, and the interpretation of results.
LIMITATIONS
PYR may be used in the presumptive separation of group A streptococci and group D enterococci from other streptococci. Additional testing, using pure culture, is recommended for complete identification.
It is important that testing first be performed to determine that the organism is Streptococcus. Only group A streptococci and group D enterococci are PYR-positive. Other streptococci are negative; however, additional testing, using pure culture, may be necessary to separate group A streptococci (S. pyogenes) from beta-hemolytic enterococci.
A gram stain and catalase test should be performed to confirm the presence of gram-positive, catalase-negative cocci. The ability to hydrolyze bile esculin may be used to presumptively identify group D enterococci.
Staphylococcus, Aerococcus, Lactococcus, most Corynebacterium haemolyticum, as well as some Enterobacteriaceae species and other gram-negative bacilli are also PYR-positive.
Most human infections associated with group D streptococci are caused by Enterococcus faecalis and Streptococcus bovis.
Refer to the keyword "Limitations", in the Hardy Diagnostics software program HUGO, for more information regarding general limitations on culture media.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, forceps, incinerators, incubators, PYR Test Kit, and/or the Esculin/PYR Screen, etc., as well as serological and biochemical reagents, are not provided.
QUALITY CONTROL
User Quality Control: Consult the appropriate technical insert (PYR Test Kit or Esculin PYR Screen) for proper quality control of the PYR Reagent. It is recommended that each new lot of reagent be tested with known positive and negative controls and retested at least each week of use thereafter.(1,5)
Physical Appearance: The PYR Reagent should appear clear to straw yellow in color.
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