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VDRL Test

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  • تصفية - فلترة
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  • VDRL Test

    Veneral Disease Resarch Laboratory(VDRL)TEST

    AIMS OF TEST
    to diagnosis of syphillis
    syphillis caused by Treponemal .pallidum
    VDRL test is screening test for the presence of reagin,antibody specific for treponemal spirochetes
    what mean with reagin
    it is mean this test does not look for antibodies against the actual bacterium,but rather fo antibodies against substances released by cells when they ar damaged byTreponema.Pallidum
    Types of VDRL test

    • VDRL-Serum
    VDRL-CSF sample obtained by lumber punctur


    principle
    heated serum or unheated CSF is mixedwith antigen to reagin on glass slide

    Antigen is purified mixture of lipid such as

    cardiolipin,lecithin,and cholestrol

    this mixture examined microscopillay to detect the clumping of the mixture

    RESULTS
    -REACTIVE(positive)clumping occure
    -NON-REACTIVE(negative)no clumping

    VDRL test usually become reactive within the first weeks after infection

    VDRL test can fail to show reactive results in the late stage of syphillis

    VDRLtest less sensitive than PRP test for primary syphillis

    false positive results are caused by the following factors
    • malaria
    • measles
    • lupus erythematosis
    • leprosis
    • hepatitis
    • Lyme diseae

    refrigeration for 72hours destoyes the treponemal spirochetes

    a person may negative VDRL test and still have syphillis inthe early stages of disease

    positive results confirmed by (FTA-Abs DS)Test
    Fluorescence Treponemal Antibody-Absorption double stain test

    this test provide the most sensetive detection of treponemal antibodies for syphillis in all stages

    in this test
    serum treated to remove antibodies that could cause false-positive results

    this techniqe involve fluorescence microscope with special filters that decrease the amount of natural fluorescence from background of the spicemen


    fluorescein-conjugated antibodies to IgG are added as counterstain to the stained the spicemen

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  • #2
    شكراً الله يعطيك العافية
    تواضع تكن كالنجم لاح لناظر على صفحات الماء وهو رفيع

    و لا تك كالدخان يعلو بنفسه على طبقات الجو و هو وضيع


    http://up1.m5zn.com/photo/2009/2/10/...okezwv.gif/gif

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    • #3
      يسلموووووووووو الله يعطيك العافية

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      • #4
        بارك الله فيك
        تحياتي
        :extra59: العلم نور :extra57:

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