المشاركة الأصلية بواسطة yfrewan
مشاهدة المشاركة
A colorimetric technique used for estimation of total protein
biurate reagent blue turn violet in the presence of protein and changed to pink when combined with short -chain polypeptides
biurate reagent used to protein assay
a-add o.5 ml of biurate reagent
b-add 10 microliter of serume
c- wait 10 minutes
d- read on spectrophotometer at 546 nm
also can use trichloroacetic acid,this acid precipitate albumne and globulin
تعليق