تويت
A P I ( Analytical Profile Index )
The Analytical Profile Index or API is a classification of bacteria based on experiments, allowing fast identification.
& it can perform at least 20 different biochemical tests ... it's primarily for the identification of Enterobacteriaceae.....
This is the types of testes & its abbreviation
1. ONPG: test for -galactosidase enzyme by hydrolysis of the substrate o-nitrophenyl--D-galactopyranoside
2. ADH: decarboxylation of the amino acid arginine by arginine dihydrolase
3. LDC: decarboxylations of the amino acid lysine by lysine decarboxylase
4. ODC: decarboxylations of the amino acid ornithine by ornithine decarboxylase
5. CIT: utilization of citrate as sole carbon source
6. H2S: production of hydrogen sulfide
7. URE: test for the enzyme urease
8. TDA: detection of the enzyme tryptophan deaminase
9. IND: production of indole from tryptophan by the enzyme tryptophanase. Indole is detected by addition of Kovac's reagent.
10. VP: the Voges-Proskauer test for the detection of acetoin (acetyl methylcarbinol) produced by fermentation of glucose by bacteria utilizing the butylene glycol pathway
11. GEL: test for the production of the enzyme gelatinase which liquefies gelatin
12. GLU: fermentation of glucose (hexose sugar)
13. MAN: fermentation of mannose (hexose sugar)
14. INO: fermentation of inositol (cyclic polyalcohol)
15. SOR: fermentation of sorbitol (alcohol sugar)
16. RHA: fermentation of rhamnose (methyl pentose sugar)
17. SAC: fermentation of sucrose (disaccharide)
18. MEL: fermentation of melibiose (disaccharide)
19. AMY: fermentation of amygdalin (glycoside)
20. ARA: fermentation of arabinose (pentose sugar)
هذه صوره ل ال API وهذه الألوان التى تروها تمثل microtubes والتى يوضع بها ال bacterial suspension وتجرى الإختبارت فى دقائق معدوده ... وفى وقتها تستطيع ان تعرف نوع ال organism وانت واقف ... مش هتحتاج وقت طوبل ...
وهذا عرض فلاشى يشرح كل الكلام اللى قلته :::
http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/shoeb/api20.html
أتمنى انكم تكونوا أستفدتم من الموضوع ...
محبتكم
طموحه جدا
& it can perform at least 20 different biochemical tests ... it's primarily for the identification of Enterobacteriaceae.....
This is the types of testes & its abbreviation
1. ONPG: test for -galactosidase enzyme by hydrolysis of the substrate o-nitrophenyl--D-galactopyranoside
2. ADH: decarboxylation of the amino acid arginine by arginine dihydrolase
3. LDC: decarboxylations of the amino acid lysine by lysine decarboxylase
4. ODC: decarboxylations of the amino acid ornithine by ornithine decarboxylase
5. CIT: utilization of citrate as sole carbon source
6. H2S: production of hydrogen sulfide
7. URE: test for the enzyme urease
8. TDA: detection of the enzyme tryptophan deaminase
9. IND: production of indole from tryptophan by the enzyme tryptophanase. Indole is detected by addition of Kovac's reagent.
10. VP: the Voges-Proskauer test for the detection of acetoin (acetyl methylcarbinol) produced by fermentation of glucose by bacteria utilizing the butylene glycol pathway
11. GEL: test for the production of the enzyme gelatinase which liquefies gelatin
12. GLU: fermentation of glucose (hexose sugar)
13. MAN: fermentation of mannose (hexose sugar)
14. INO: fermentation of inositol (cyclic polyalcohol)
15. SOR: fermentation of sorbitol (alcohol sugar)
16. RHA: fermentation of rhamnose (methyl pentose sugar)
17. SAC: fermentation of sucrose (disaccharide)
18. MEL: fermentation of melibiose (disaccharide)
19. AMY: fermentation of amygdalin (glycoside)
20. ARA: fermentation of arabinose (pentose sugar)
هذه صوره ل ال API وهذه الألوان التى تروها تمثل microtubes والتى يوضع بها ال bacterial suspension وتجرى الإختبارت فى دقائق معدوده ... وفى وقتها تستطيع ان تعرف نوع ال organism وانت واقف ... مش هتحتاج وقت طوبل ...
وهذا عرض فلاشى يشرح كل الكلام اللى قلته :::
http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/shoeb/api20.html
أتمنى انكم تكونوا أستفدتم من الموضوع ...
محبتكم
طموحه جدا
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