the test can be done by inoculate pure culture of candida in 1ml of serum then incubate at 37c in incubator at least 2 Hours
then add drop from will mixing serum in slide and cover by coverslide then examine by 40x
if the long tube seen beside the yeast cell that mean candida albicans and this tube called germ tube
if the cells seen without any budding that mean other candida species
Germ tube test is method to identification of candida albicans by it production of filaments or germ tube by low concentration of cells of c.albicans in serum tube
_In 0.5 ml of human or sheep serum give rapid presumptive identification within tow or three hours with control at 37c
Factor can prevent germ tube production :
• High concentration of the yeast cell .
• Temperature of incubation more than 41 c or below of 31 .
• Heat coagulated serum .
• Freash and inactive human serum .deep freez sheep serum .
:extra4:سعيد ذلك الإنسان الذي يترك خلفه ظلالا من الحب :extra57:
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