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|[ coagulase ]|

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  • |[ coagulase ]|

    السلام عليكم ورحمة الله وبركاااته ..

    شخبآركم أن شاء الله بخيــر

    انا عندي سؤال بسيط وأتمنى الاجابه عليه .. وشاكراًُ لكم


    what is the difference between the coagulase slide test and coagulase tube test ?

    س / مالفرق بين أختبآر coagulase في slide وأختباره في tube ؟

    ولا تحرموني من الاجابة - والله ولي التوفيق

    التعديل الأخير تم بواسطة عمآد الغآمدي; الساعة 07-11-2009, 08:52 PM.

  • #2
    اللي في ال slide اسمه bound coagulase وهي طريقة سريعة تتم في ثواني
    واللي في ال tube اسمه free coagulase وتاخذ من ساعة لاربع ساعات ويتم وضع الtube في ال incubator
    وهذا باختصار اتمنى اكون وضحت المعلومة

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    • #3
      كآن هذا جوابي لدكتور

      للآسف أجابتك ماهي كامله .

      لكن أبي الميزات فيما بينهم

      أتمنى الرد

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      • #4
        معقوله الى الان مآحد عرف اجابة هالسؤال ؟:sm205:

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        • #5
          SLIDE COAGULASE TEST:
          Principle: The bound coagulase is also known as clumping factor. It cross-links the α and β chain of fibrinogen in
          plasma to form fibrin clot that deposits on the cell wall. As a result, individual coccus stick to each other and
          clumping is observed.
          Procedure: Dense suspensions of Staphylococci from culture are made on two ends of clean glass slide. One
          should be labeled as “test” and the other as “control”. The control suspension serves to rule out false positivity due
          to autoagglutination. The test suspension is treated with a drop of citrated plasma and mixed well. Agglutination or
          clumping of cocci within 5-10 seconds is taken as positive. Some strains of S.aureus may not produce bound
          coagulase, and such strains must be identified by tube coagulase test.
          TUBE

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          • #6
            TUBE COAGULASE TEST:
            Principle: The free coagulase secreted by S.aureus reacts with coagulase reacting factor (CRF) in plasma to form
            a complex, which is thrombin. This converts fibrinogen to fibrin resulting in clotting of plasma.
            Procedure: Three test tubes are taken and labeled “test”, “negative control” and “positive control”. Each tube is
            filled with 0.5 ml of 1 in 10 diluted rabbit plasma. To the tube labeled test, 0.1 ml of overnight broth culture of test
            bacteria is added. To the tube labeled positive control, 0.1 ml of overnight broth culture of known S.aureus is added
            and to the tube labeled negative control, 0.1 ml of sterile broth is added. All the tubes are incubated at 37oC and
            observed up to four hours. Positive result is indicated by gelling of the plasma, which remains in place even after
            inverting the tube. If the test remains negative until four hours at 37oC, the tube is kept at room temperature for
            overnight incubation.

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            • #7
              أشكرك وبعمق .. لكن مافي رد أقرب من كذا وأوضح وبإختصار


              ومقدر لك تعبك هذا ولا أنسى لك جميلك

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