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**caesar22** · 06-11-2010, 10:34 PM

Immunoblot

The Western blot (alternatively, protein immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.

http://en.wikipedia.org/wiki/Western_blot

**abuyafa** · 22-12-2010, 11:12 PM

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تقنية الـ Recombinant immunoblot assay

تقنية الـ Recombinant immunoblot assay

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