تويت
السلام عليكم ورحمة من الله وبركاته
هذه معلومات بسيطة عن هذا الاختبار :-
هو اختصار لـ :- C= Christie
A=Atkinase
M=Munch
P=petrson
CAMP Test
1. Obtain a sheep blood agar plate that has been prepared for a CAMP test by
having Staphylococcus aureus streaked in a single line down the center of the
plate.
2. Lines have been drawn on the plate perpendicular to the Staph. streak. These
will act as guidelines for inoculating the plate. Label one of the lines on the
CAMP plate with the organism to be tested.
3. Using a sterile loop obtain a sample of the test organism. Using a single streak
and moving from the outer edge of the CAMP plate toward the Staph. steak,
inoculate the CAMP plate with the test organism. Do not allow the test organism
to directly touch the Staph. streak or streak across the Staph. streak. The test
organism should be streaked using one of the perpendicular lines as a guide.
4. Invert the plate and place it in the incubator for a minimum of 18 hours.
5. Observe the plate for the development of a distinct arrowhead pattern of
hemolysis where the test organism and the Staph. almost touch.
6. Discard the plate in the biohazard container.
Interpretation: The arrowhead hemolysis pattern is considered positive (+) for this test. No hemolysis or indistinct hemolysis patterns are considered negative (-) for this test.
*This test should be done only on organisms that display beta or gamma hemolysis.
وهي تستخدم : STREPTOCOCCI spp
يعني على البلد اقار تحط خط من فوق الى تحت
وبعدين اربع نقاط ثنتين على اليمين وثنتين على اليسار من الخط بدون ملامسته وتخليها في الحضانه لمدة 24 ساعه وتشوف النتيجه اذا التصقت يعني بوزتف + واذا هي نفس ما تركتيها فهي نيقتف -
وهذه صورة لعينه بوزتف في المستند حالياً.
هذه معلومات بسيطة عن هذا الاختبار :-
هو اختصار لـ :- C= Christie
A=Atkinase
M=Munch
P=petrson
CAMP Test
1. Obtain a sheep blood agar plate that has been prepared for a CAMP test by
having Staphylococcus aureus streaked in a single line down the center of the
plate.
2. Lines have been drawn on the plate perpendicular to the Staph. streak. These
will act as guidelines for inoculating the plate. Label one of the lines on the
CAMP plate with the organism to be tested.
3. Using a sterile loop obtain a sample of the test organism. Using a single streak
and moving from the outer edge of the CAMP plate toward the Staph. steak,
inoculate the CAMP plate with the test organism. Do not allow the test organism
to directly touch the Staph. streak or streak across the Staph. streak. The test
organism should be streaked using one of the perpendicular lines as a guide.
4. Invert the plate and place it in the incubator for a minimum of 18 hours.
5. Observe the plate for the development of a distinct arrowhead pattern of
hemolysis where the test organism and the Staph. almost touch.
6. Discard the plate in the biohazard container.
Interpretation: The arrowhead hemolysis pattern is considered positive (+) for this test. No hemolysis or indistinct hemolysis patterns are considered negative (-) for this test.
*This test should be done only on organisms that display beta or gamma hemolysis.
وهي تستخدم : STREPTOCOCCI spp
يعني على البلد اقار تحط خط من فوق الى تحت
وبعدين اربع نقاط ثنتين على اليمين وثنتين على اليسار من الخط بدون ملامسته وتخليها في الحضانه لمدة 24 ساعه وتشوف النتيجه اذا التصقت يعني بوزتف + واذا هي نفس ما تركتيها فهي نيقتف -
وهذه صورة لعينه بوزتف في المستند حالياً.