Acanthamoeba keratitis(AK) is infection of the human eye cornea by the free –living protozoan Acanthamoeba whish is cause blindness hn man , ,the main risk factors associated with (AK) are eye trauma and cotact lens wear .
Patients typically suffer from atriad of sever ocular pain ,photophobia ,and aunilateral red eye ,,, laboratory confirmation of (AK) is usually made by
Microscopy examination
The corneal swab or scraping was suspended in 10ml of 0.9% nacl , centrifuged at medium speed (400 rpm) for 5 min , most of supernatent was aspirated and a smear from the sediment is prepared and then examined microscopically .
Culture
The corneal swab or scraping was suspended in 10ml of 0.9% nacl most of suspended was aspirated and the sediment resuspended in remaining fluid . using a sterile pasteuer pipette two or three drops of the suspension was placed in the center of the agar plate seeded with E.coli bacteria incubated at 30 - 35c and examined for characteristic Acanthamoeba trophozoite .
Patients typically suffer from atriad of sever ocular pain ,photophobia ,and aunilateral red eye ,,, laboratory confirmation of (AK) is usually made by
Microscopy examination
The corneal swab or scraping was suspended in 10ml of 0.9% nacl , centrifuged at medium speed (400 rpm) for 5 min , most of supernatent was aspirated and a smear from the sediment is prepared and then examined microscopically .
Culture
The corneal swab or scraping was suspended in 10ml of 0.9% nacl most of suspended was aspirated and the sediment resuspended in remaining fluid . using a sterile pasteuer pipette two or three drops of the suspension was placed in the center of the agar plate seeded with E.coli bacteria incubated at 30 - 35c and examined for characteristic Acanthamoeba trophozoite .
تعليق