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Aseptic Technique

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  • Aseptic Technique



    Aseptic Technique

    This subject is involve in the use of sterile media in culture tubes and Petri plates. The proper handling of these materials requires special skills that you must master. It is the purpose of this exercise to provide you with procedures that will become routine as you progress through this course. It’s all about aseptic technique.


    Aseptic transfer of a culture from one culture vessel to another is successful only if no contaminating microorganisms are introduced in the process. A transfer may involve the transport of organisms from an isolated colony on a plate of solid medium to a broth tube, or inoculating various media (solid or liquid) from a broth culture for various types of tests.


    To gain some practice in aseptic transfer of bacterial cultures, three simple transfers will be performed here in this exercise: (1) broth culture to broth, (2) agar slant culture to agar slant, and (3) agar plate
    to agar slant. Proceed as follows:


    TRANSFER FROM BROTH CULTURE TO ANOTHER BROTH

    Do a broth tube to broth tube inoculation, using the following technique. Figure 1 illustrates the procedure for removing organisms from a culture, and figure 2 shows how to inoculate a tube of sterile broth.





    TRANSFER OF BACTERIA FROM SLANT TO SLANT

    To inoculate a sterile nutrient agar slant from an agar slant culture, use the following procedure. Figure 4 illustrates the entire process.





    WORKING WITH AGAR PLATES


    (Inoculating a slant from a Petri plate)

    The transfer of organisms from colonies on agar plates to slants or broth tubes is very similar to the procedures used in the last two transfers (broth to broth and slant to slant). The following rules should be observed.



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