he Vitros 250, Chemistry Analyzer
The Vitros 250 is a chemistry analyzer capable of measuring 36 analytes on a sample.
• Procedure
Collect blood from mice using at least 22-gauge needle to avoid hemolysis. Serum can be obtained by allowing the blood to clot at room temperature for 30 minutes and then centrifuging at 4000 rpm for 10 minutes, or by using serum separator tubes that can be purchased from Becton Dickenson (order number 365956, vendor: J J Nita Burgoon Co.). Samples that have been collected in EDTA tubes (plasma) cannot be used.


• Volume required
40ul of serum plus 10ul for each analyte measured is required. Samples should be brought to room L5.122 on wet ice or frozen (note: ALT measurements from frozen samples are not reliable). Please label all samples sequentially with a study number.
Raw data provided in paper format or through e-mail as a PDF file.


• Tests the machine is capable of running:
Albumin (ALB) Carbon Dioxide (ECO2)
Alcohol (ALC)* Iron (Fe)*
Alkaline Phosphatase (ALKP) Gamma Glutamyltransferase (GGT)
Alanine Aminotransferase (ALT) Glucose (GLU)
Ammonia (AMON)* Potassium (K)
Amylase (AMYL)* Lactate (LAC)*
Aspartate Aminotransferase (AST) Lactate Dehydrogenase (LDH)
Unconjugated/Conjugated Bilirubin (BuBc)* Lipase (LIPA)
Blood Urea Nitrogen (Bun) Magnesium (Mg)*
Calcium (Ca) Sodium (Na)
Cholinesterase (CHE)* Phosphorus (PHOS)
Cholesterol (CHOL)/(HDLC) CSF Protein (PROT)*
Creatine Kinase (CK) Total Bilirubin (TBIL)
Creatine Kinase MB (CKMB)* Total Protein (TP)
Chloride (Cl) Triglyceride (TRIG)
Creatinine (CREA) Urine Protein (UPRO)*
C-Reactive Protein (CRP)* Uric Acid (URIC)*
Direct HDL (dHDL)

Axsym
The Abbott Axsym is an immunochemical automated analyser made by Abbott Laboratories. It is used for serology tests and therapeutic drug monitoring, and uses antibodies to alter the deflection of polarised light. It can also be used to monitor hormone level and some cardiac markers such as troponin.
Appearance and use
Samples and reagents go in the carousel on the right of the machine. This instrument is utilized in medical laboratorys by medical technologists.