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Benedict’s Test

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  • Benedict’s Test

    benedects test ready by samy khwaiter.JPGBenedict’s Test:
    Principle:
    Benedict test is Specific to reducing sugars, sugars with a free aldehyde or ketone group) such as Fructose, Glucose, Galactose, Lactose & Maltose. It is used to differentiate between non-reducing (e.g. Sucrose) & reducing sugars.
    Benedict is formed from sodium carbonate + sodium citrate dihydrate + copper sulfate pentahydrate. The copper sulfate (CuSO4) present in Benedict's solution reacts with electrons from the aldehyde or ketone group of the reducing sugar to form cuprous oxide (Cu2O), of yellowish orange or red ppt.



    Procedure & observation:
    - To 2 mL of carbohydrate solution in a test tube, add 1 mL of Benedict reagent.
    - Mix well, and boil it up for 2 min
    - Observe yellowish orange or red ppt.

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    Samy Khwiter
    Biomedical Lecturer

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    Samy Khwiter
    Biomedical Lecturer

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