تويت
ISOLATION OF VIRUSES "عزل الفيروسات"
ISOLATION OF VIRUSES USE
1_TISSUE AND CELL CULTURE
2_EMBERYONATED EGG
3_ANIMAL INOCULATION
PURPOSE OF VIRAL ISOLATION
1_DIAGNOSIS
2_RESEARCH
3_PRODUCTION OF ANTIGEN AND VACCINE
CELL CULTURE
CELLS OBTAINED BY DISSOCIATION OF ORGANS CHOPPED " CUT
FOLLOWED BY TREATMENT BY ENZYMES LIKE TRYPSIN OR COLLAGENASE TO GIVE INDIVIDUAL CELL
THESE INDIVIDUAL CELLS GROWING IN CONTAINER CELL CULTURE FLASK
MEDIA USE FOR GROWING CELLS
1_GROWTH MEDIA
THIS BROTH MEDIA CONTAIN FACTORS MAKE CELL LIFE LIKE AMINO ACID ,GLUCOSE ,VITAMINS ,FOETAL CALF SERUM AT CONCENTERATION 10_20% , PHENOL RED INDICATOR AND ANTIBIOTEC
2_MAINTENANCE MEDIA
LIKE GROWTH MEDIA BUT WE DEPRESS THE CONCENTERATION OF FOETAL CALF SERUM TO 2_5% BECAUSE NO NEED FOR MORE MULTIBLICATION OF CELLS
CULTURE FLASK
:more20::more20::more20:
1_TISSUE AND CELL CULTURE
2_EMBERYONATED EGG
3_ANIMAL INOCULATION
PURPOSE OF VIRAL ISOLATION
1_DIAGNOSIS
2_RESEARCH
3_PRODUCTION OF ANTIGEN AND VACCINE
CELL CULTURE
CELLS OBTAINED BY DISSOCIATION OF ORGANS CHOPPED " CUT
FOLLOWED BY TREATMENT BY ENZYMES LIKE TRYPSIN OR COLLAGENASE TO GIVE INDIVIDUAL CELL
THESE INDIVIDUAL CELLS GROWING IN CONTAINER CELL CULTURE FLASK
MEDIA USE FOR GROWING CELLS
1_GROWTH MEDIA
THIS BROTH MEDIA CONTAIN FACTORS MAKE CELL LIFE LIKE AMINO ACID ,GLUCOSE ,VITAMINS ,FOETAL CALF SERUM AT CONCENTERATION 10_20% , PHENOL RED INDICATOR AND ANTIBIOTEC
2_MAINTENANCE MEDIA
LIKE GROWTH MEDIA BUT WE DEPRESS THE CONCENTERATION OF FOETAL CALF SERUM TO 2_5% BECAUSE NO NEED FOR MORE MULTIBLICATION OF CELLS
CULTURE FLASK
:more20::more20::more20:
تعليق