تويت
(Method for slide test (to detect bound coagulase
-.place a drop of physiological saline on each end of a slide
Take a colony of the test organisms in each of the drops to make two thick suspensions -
Add a drop of plasma to one of the suspensions , and mix gently . look for clumping of the organisms within 10 seconds
No plasma is added to the second suspension .
This is used to differentiate any granular appearance of the organism from true coagulase clumping .
Results
Clumping within 10 seconds................S.aureus
No clumping within 10 seconds ...........No bound coagulase produced
-.place a drop of physiological saline on each end of a slide
Take a colony of the test organisms in each of the drops to make two thick suspensions -
Add a drop of plasma to one of the suspensions , and mix gently . look for clumping of the organisms within 10 seconds
No plasma is added to the second suspension .
This is used to differentiate any granular appearance of the organism from true coagulase clumping .
Results
Clumping within 10 seconds................S.aureus
No clumping within 10 seconds ...........No bound coagulase produced
تعليق