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  • دعوة للتفاعل

    هذة دعوة للجميع ليتفاعل مع الموضوع والذى ساتحدث عنة
    ساطرح موضوعى هذا بصيغة السؤال ولن نكتفى بالاجابات المحددة بل من اراد الاجابة فلة ان يوضح الاجابة ان اراد وان يضيف لموضوع السؤال ان احب
    الموضوع سيتطرق الى Biochemical reaction of bacteria
    OUESTIONS

    1- mention three test used to identify s.aures
    a------------
    b------------
    c-------------
    2-test used to differntiate between staphyllococcus and streptococcus------------which give positive with---------spp
    3-bile solubility test used to differntiat between-----------an viradins streptococci
    4-many test used to differntiate enterobacteriacea spp. mention two of them
    a---------------
    b---------------
    Test used to differntiate between BACTEROIDES spp.andBRUCELLA
    6-The test used to differntiate between proteusand other Enterobacteriacea spp--------test
    7-In Indol test the E.coli have the ability to breakdown --------------amino acid into indol
    8-to identification of Enterococci spp.used------test
    9-Oxidase test used to identification of the following bacteria
    a------------
    b----------
    c---------

    10-to differntiated between s.pneumonia and alph hemolytic- streptococci using--------disk
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  • #2
    :sm200: لا لا لا مستحيــــــــل !!! :sm202:

    هذه الأسئلة تحتاج ساعات من الشرح !!!

    أنا من شوي خلصت الإجابة في موضوع "لننعش معلوماتنا من جديد"

    بس ولا يهمكم... أنا في عطلة منتصف السنة الدراسية حاليا ... وسأكون أكثر من سعيدة بالمشاركة...

    جــــــزاك الله كـــــل خيـــــر

    :sm188: لي عودة إن شاء الله :sm188:

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    • #3
      بسم الله الرحمن الرحيم وبه نستعين

      سأقوم بالرد بشكل مختصر جدا عن الأسئلة التي أعرف إجاباتها:

      mention three tests used to identify S. aureus
      Catalase
      Coagulase
      DNASE
      S. aureus gives a positive restult for all the previous tests


      The catalase test is used to differenciate between Staphylococci and Streptococci
      Staphylococci spp. give positive result for catalase, while Streptococci spp give negative result


      I could not understand the third question


      To differenciate between Entesriobacteraceae, many tests are used... SUCH AS:
      Indole test
      Urease test
      Motility Indole Orthinin test
      Triple Sugar Iron Agar
      Simmons Citrate Agar
      API 20 E to identify the species


      I am Sorry BUT i do not know the answer for question 5


      The only bacteria that can give Swarming growth is Proteus spp. So swarming growth can be used to identify Proteus between all the Enteriobacteriaceae


      الأسئلة 7 و 8 و 9 أحتاج فيهم للمساعدة !!!


      We can differntiate between s.pneumonia and alph hemolytic- Streptococci we can use the Optechin disk... S. pneumonia is Susceptable to the disk

      أتمنى أن تكون إجاباتي صحيحة :sm185:

      إذا كانت صحيحة أكون أخذت خمسة من 10 :sm196: :sm195:

      :sm171: خير إن شاء الله :sm171:

      جزاك الله عنا كل خير

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      • #4
        ouestion no .6
        the test is urease test


        principle of test
        the test organism is cultured in a medium which contain urea and the indicator phenol red, if the strain is ureas-producing,the enzyme will breakdown the urea togive ammonia and carbon dioxide
        with release of ammonia,the medium becomes alkaline as shown by a chang in colour of the idicator to red-pink


        RESULTS

        RED- PINK MEDIUM-------POSITIVE TEST(UREASE PRODUCED

        NO RED-PINK COLOUR------NEGATIVE RESULTS(NO UERASE PRODUCED

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        • #5
          اختى العزيزة مختبرى مملكتى اجابتك كلها صحيحة ولكن بالنسبة للسؤال السادس كلامك صحيح عنSWARMING OF PROTUSE ,ولكن نريد biochemical test

          اشكرك على هذا التفاعل الرائع واتمنى مشاركة الجميع


          بالنسبة للاسئلة التى تمت الاجابة عليها ليس معنى هذا انة انتهى الموضوع اى بمعنى انة فى السؤال الاول ليتحدث احدنا على coagulase test واخريتحدث عن catalase test وهكذا حتى يتكامل الموضوع

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          • #6
            السؤال الثالث
            bile solubility test فحص يستخدم للتفريق بين viridans streptococciو--------------


            اتمنى يكون السؤال واضح اختى العزيزة
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            • #7
              1- mention three test used to identify s.aures
              a--DNase-
              b-coagulase-----
              catalase----
              2-test used to differntiate between staphyllococcus and streptococcus-catalase which give positive with-staph spp
              3-bile solubility test used to differntiat between-S.pneumoniae-an viradins streptococci
              4-many test used to differntiate enterobacteriacea spp. mention two of them
              a-indoe-
              b-urease
              .........Test used to differntiate between BACTEROIDES spp.andBRUCELLA
              6-The test used to differntiate between proteusand other Enterobacteriacea spp-urease-test
              7-In Indol test the E.coli have the ability to breakdown tryptophan-amino acid into indol
              8-to identification of Enterococci spp.used-esculine hydrolysis test-----test
              9-Oxidase test used to identification of the following bacteria
              avibrio---
              pseudomonas-
              c---------

              10-to differntiated between s.pneumonia and alph hemolytic- streptococci using-optechin disk

              انا شاء الله تكون صحيحه:sm194:
              [http://img06.picoodle.com/img/img06/...rm_bf2fcec.jpg

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              • #8
                ساتحدث عن Indole Test

                principle
                the test organismis cultured in amedium which contain tryptophan.indole production is detected by Kovac,s or Ehrlich,s reagent,this react with the indole to produce a red coloured compound
                RESULTS



                اخى tiger اشكرك على مشاركتك الرائعة

                يا ريت تحدثنا عن احد الفحوصات

                اتمنى من الجميع المشاركة


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                • #9
                  السؤال الاول الاجابات صحيحة تماما اضيف اليها فحص اخر وهو gelatinaze test وساتحدث عنة اليوم.
                  gelatinaze test
                  purpose
                  to differntiate between the following bacteria
                  1-S.aures andS.epidermides
                  2-serratia.marcescens and protus.vulgaris
                  principel

                  nutrient gelatine is adifferntial medium that test the ability of an organism to produce an exoenzyme called gelatinaze, that hydrolyses gelatine
                  gelatine is protein derived from connective tissu, when temperature of gelatine is below 32C it is semisolid,while when atemperature above 32C it is aviscous liquid

                  the organism that produce gelatinaze enzyme have the ability to breakdown gelatine into smaller polypeptide,peptide and amino acid that cross the cell membrane and be utilized by the organisms
                  s.marcescens is positive for gelatinaze production
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                  • #10
                    Bile Esculine Test
                    purpose
                    to identify bacteria able to hydrolyze esculin in the presence of blie

                    all of thes bacteria are positive for bile esculin
                    1-group D streptococci

                    2-Enterococci as E.faecalis,E.faecium,E.bovis


                    media contain the following

                    esculin is aglycoside(composed of glucose and esculetin)
                    peptone for nutrition
                    bile to inhibit gram-positive bacteria other than enterococci and group D streptococci
                    ferric citrate (indicator colour

                    ferric citrate react with esculetin to form dark colour

                    principle

                    the organism split the esculin into glucose and esculetin ito the medium
                    the esculetin react with ferric citrate in the medium to form a phenolic iron complex compound which turn the agar slant dark brown to black



                    A B


                    A=S.pyogenes does not hydrlyze the esculin,negative result

                    B=E.faecalis hydrolyze esculin,positive result
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                    • #11
                      حديثنا اليوم عن

                      OXIDASE TEST
                      purpose
                      to identify the bacteria that have the ability to produce oxidase enzyme such as
                      • psudomonas
                      • neisseria
                      • vibrio
                      • pasturella
                      • moraxella
                      principle
                      a piece of filter paper is soaked with afew drops of oxidase reagent(tetramethyl-phenylenediamine dihydrochloride)
                      acolony of the test organism is then smeared on the filter paper

                      IF the organism is oxidase producing, phenylenediamine in the reagent will be oxidized to deep purple colour
                      NOTES
                      1. flooding the culture plate with oxidase reagent occasionally used but this technique is not recommended for routine use because the reagent rapidly kills bacteria
                      example
                      to isolate N.gonorrhoeae from mixed culture,the oxidase positive colonies must be removed and subcultured within 30 seconds of flooding the plate
                      2-- acidity inhibits oxidase enzyme activity
                      3-- the oxidase test must not be performed on colonies that produce fermantation on carbohydrate-containing media because this prevent the reaction from occuring
                      example
                      V.cholera colonies on TCBS medium ferment sucrose
                      so subinoculation on nutrient agar is required before the oxidase test can be performed
                      so Non-fermenting colonies can be tested

                      4--colonies tested from a medium that contains nitrate may give unreliable results

                      5--to remove the colonies from culture use glass rod or a piece of stick but not use wire loop

                      6---results from old culture are unreliable
                      7---you must use bacteria from a culture grown on T-soy agar for 24-36 hour


                      RESULTS

                      postive results blue-purple clour
                      oxidase produced
                      negative results no colour
                      no oxidase produced

                      Note
                      ignore any blue-purpule colour that
                      develops after 10 seconds


                      control

                      positive control---pseudmonase.aeruginosa


                      negative control---E.coli


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                      • #12
                        citrate utilization test
                        purpose
                        to differntiated between enterobacteriacea
                        this test based on the ability of organisms to use citrate as its only source of carbon and ammonia as its only source of nitrogen

                        principle
                        the organism that cultured on the mediume utilize citrate as their sole carbon source use citrase enzyme or citrate-permease to transport the citrate into the cell
                        also convert the ammonium dihydrogen phosphate to ammonia and ammonium hydroxide ,which create the alkaline enviroment of the medium

                        at pH 7 or above the idicator bromo-thymol blue convert into blue colour at neutral pH
                        NOTE
                        the idicator is green colour


                        RESULTS
                        positive results--------blue colour
                        citrate utilized

                        example
                        salmonella.typhimuirm

                        negative results--------------no growth
                        citrate not utilized
                        Example
                        E. COLI

                        MEDIA
                        • Koser,s citrate agar
                        • Simmon,s citrate agar
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                        • #13
                          لا يسعني الاّ أن أشكرك أخي عوني يوسف وبالفم الملآن.
                          بالتوفيق.

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                          • #14
                            المشاركة الأصلية بواسطة طبيبة مختبر مشاهدة المشاركة
                            لا يسعني الاّ أن أشكرك أخي عوني يوسف وبالفم الملآن.
                            بالتوفيق.

                            لا شكرعلى واجب
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                            • #15
                              اشكرك اخي الغالي والشكر قليل في حقك صراحه معلومات قمة في الروعة
                              الحياة مليئة بالحجارة..فلا تتعثر بها بل اجمعها وابن بها سّلما تصعد به نحو النجاح

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