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Bence Jones Protein

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  • Bence Jones Protein

    السلام عليكم
    ارجو بيان طريقة الكشف عن Bence Jones Protein في البول بطريقة اضافة nitric acid.
    لانني ملتح جدا لمعرفتها

  • #2
    Bence-JonesProtein test

    Principle: Bence-Jones protein is soluble in urine at room and body temperature. When the urine is heated to 40°C
    a white cloud appears and at 60°C, a distinct precipitate forms. The precipitate disappears on boiling but reappears
    on cooling. Excessive amounts of acid or salt will prevent the appearance of the precipitate.

    Reagent.: Acetic Acid, approximately 10% aqueous solution

    Procedure. : Add urine to a 6-inch test tube until it is about two-thirds full. Place in a water bath and heat slowly. Do
    not allow the bottom of the test tube to touch the bottom of the beaker, as it may become hotter than the rest of the water
    Suspend a thermometer in the water bath. Note the temperature and the appearance of the urine every few minutes
    especially between the temperatures of 40°C - 60°C. If a slight cloud appears when the urine is heated to the boiling point, 100°C
    add a few drops of acetic acid. This will dissolve any phosphates that have separated out. If a precipitate forms at the boiling point
    it is due to albumin. Boil the urine with a few drops of dilute acetic acid, filter rapidly, and repeat the test on the cooled filtrate

    Interpretation: Bence-Jones protein is found in urine in many cases of multiple myeloma
    osteogenic sarcoma, osteomalacia, carcinomatosis
    كن حذرا وأنت تقرأ كتب الصحة، فقد تموت بخطأ مطبعي ( مارك توين)

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    • #3
      *Red Rose *

      مميـــــــــــــــــــــــــــــزه وهذا موغريب عليكِ يسلموووووووووا
      تغيبُ
      (وغيبتكٌ) مآلهآً :آسبـْآبٌ
      وشً ذنبٌ

      ..قلبَ من؛فرآقكـْ -توفيُ
      آن كـٌآآنْ
      قصدكَ ..بسُ تلعبٌ [آآرتــْـَـَـَآآح]
      ترآآآ الزمــُن ,...\كفي ووفيٌ.

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      • #4
        Bence Jones protein analysis
        Introduction:

        Bence Jones protein is A special protein. It`s concentration increase in urine in dangerous disease cases such as bones , leukemia & liver disease.

        Procedure:

        Put 5ml from urine sample in glass test tube.
        Put the test tube in water bath at: 56C for 30min.
        Add 10 drops of dilute acetic acid. If the sample still clear after this time the result is negative.
        If the sample is turbid after 30 min , we must rise the water bath temperature to 100 C & leave the sample for another 30 min. If the turbid is still there , the result is positive
        & if the turbid is dissolved the result is negative.
        :sm194::sm194::sm194:

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