اللي عندو معلومات عن الـ c3 و c4 .....

[اخصائي احياء دقيقه]

اتمنى انكم تساعدوني

اي احد عندو معلومات عن قياس ال c3 و c4 واي معلومات عن الاجهزه المستخدمه ياريت ما تبخلو على اخوكم..

وشاكر لكم..

[azozi]

أهلا اخي الكريم ..تفضل هذا الرابط سيساعدك على استخراج معلوماتك

http://www.arabslab.com/vb/showthread.php?t=2422

[اخصائي احياء دقيقه]

مشكوووووووووور اخوي وما قصرت بس انا ابي معلومات عمليه

يعني بالمختبر وبالاخص عن الاجهزه المستخدمه والطرق التي يتم بيها قياس الc3و c4

وشاكر لك مره ثانيه...

[soma]

hello
this is what i found 4 u i hope it 'll help u

Laboratory
Chemical Pathology

C3 Third Component of Complement

Container:
SST tube

Normal Volume:
0.5 mL serum

Collection

Collect specimen in an SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate up to 1 week or freeze for longer storage. Assay cannot be performed on fluids. Lipemic serum is unacceptable.

Onsite Collection
Collect specimen in an SST tube. Assay cannot be performed on fluids. Cause for Rejection: Lipemic serum, more than one specimen received in a 24 hour period.

Days Set Up
Daily

Analytic Time:
8 hours

Reference Range
Adult: 83-240 mg/dl

Test Usage:
Quantitation of C3 is used to detect individuals with inborn deficiency of this factor or those with immunologic disease in whom complement is consumed at an increased rate. Increased levels are found in numerous inflammatory conditions as an acute phase reactant. Acquired deficiency is encountered in active lupus and glomerulonephritis. May be decreased in immune complex disease, serum sickness, chronic active hepatitis, inborn C3 deficiency and subacute bacterial endocarditis (SBE).

Test Limitations
Detects both biologically active and inactive C3.
Test Methodology:

Immunoturbidimetric
Additional Information:

C3 comprises about 70% of the complement system and is utilized in both the classical and alternate pathways of complement activation. Increased levels are found in numerous inflammatory states as an acute phase reactant. CH50 (total complement hemolytic activity), C3 and/or C4 may be decreased in cases of systemic lupus erythematosus, especially in cases with lupus nephritis, in acute and chronic hypocomplementemic nephritis, SBE, disseminated intravascular coagulation (DIC), and partial lipodystrophy (with associated nephritis-like activity in serum). Cases of hereditary C3 deficiency, while rare, have been reported and are characterized clinically by recurrent infections (e.g., pneumonia, meningitis, impetigo). Low C3 levels have also been found in cases of uremia, chronic liver diseases, anorexia nervosa, and coeliac disease.









Laboratory:
Chemical Pathology

Complement C4 Fourth Component of Complement
Container:

SST tube
Normal Volume:

0.5 mL serum
Minimum Volume:

0.25 mL serum
Offsite Collection

Collect specimen in an SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate up to 1 week or freeze for longer storage. Assay cannot be performed on urine or fluids. Lipemic serum is unacceptable.
Onsite Collection

Collect specimen in an SST tube. Assay cannot be performed on urine or fluids.
Days Set Up:

Daily
Analytic Time:

8 hours
Reference Range:

Adult: 13-60 mg/dl
Test Usage:

C4 is decreased when classical pathway of complement is hyperactivated. It is usually decreased in hypergammaglobulinemia, cryoglobulinemia, inborn C4 deficiency, active SLE, serum sickness, SBE, chronic active hepatitis, acute glomerulonephritis and rheumatoid arthritis, as well as in hereditary angioneurotic edema.
Test Methodology:

Immunoturbidimetric
Additional Information:

C4 comprises approximately 20% of the complement system. C4 is utilized only by the classical pathway, so that it is decreased only when this arm is hyperactivated. In diseases activating the alternate pathway alone, C4 levels will be normal. Total hemolytic activity (CH50), C3, and C4 are frequently decreased in a variety of conditions relating to immune complex formation. While many complement components are depressed with uremia, C4 is an exception and is elevated with uremia.

Good Luck

[اخصائي احياء دقيقه]

الله يعطيكي الف عافيه اختي سوما

وماقصرتي كفيتي ووفيتي...

والله يعين على الترجمه.... ههههه

شاكر لك جهدك وتواصلك ..

[soma]

u r welcome

good luck