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CROSSMATCH PROCEDURE-GEL METHOD

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  • CROSSMATCH PROCEDURE-GEL METHOD


    CROSSMATCH PROCEDURE-GEL METHOD
    The crossmatch compatibility test is used to detect the presence of blood group antibodies in an intended
    recipient’s serum/plasma directed toward antigens present on donor red blood cells.

    PROCEDURE
    1- Label a test tube for each donor to be tested

    2- Dispense 0.5mL of Diluent 2 into the labeled tubes.

    3- add 10uL of the donor packed red blood cells from the segment to the tube of Diluent 2 and Mix gently.

    4- Label the gel Card with the appropriate identification and test information

    5- add 50uL of each donor unit 0.8% red blood cell suspension to the correct microtube. Do not touch pipette to gel card.

    6- add 25uL of patient serum or plasma to the correct microtubes.

    7- Incubate at 37° +/- 2° C for 15 minutes

    8- Centrifuge the gel card for 10 minutes.

    9- Read the front and the back of each microtube


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