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BLOOD GROUP BY GEL MATHOD

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  • BLOOD GROUP BY GEL MATHOD

    BLOOD GROUP BY GEL MATHOD
    PROCEDURE
    ABO Forward Grouping
    1- Label a clean tube

    2- Dispense 0.5mL of Diluent 2 into the labeled tubes.




    3- Add 50 μL of whole blood OR 25 μl packed red blood cells to the tube of Diluent 2 and Mix gently.

    4- Label the gel card with the appropriate sample identification

    5- Add 10-12.5 µL of 4% ±1% red blood cells diluted in Diluent 2 to the Anti-A, Anti-B, Anti-D and Control microtubes.

    Reverse Grouping

    6- Add 50 µL of each of the 0.8% suspensions of reagent A1 and B red blood cells to the labeled Buffered Gel microtubes.

    7- Add 50 µL of serum or plasma to the reagent A1 and B red blood cells in the Buffered Gel microtubes

    8- Centrifuge the gel card in the Centrifuge

    9- Reading observe the front and the back of each microtube macroscopically and record reactions

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