تويت
السلام عليكم ورحمة اللة وبركاتة
\\FLUID CLTURE MEDIA
They are many types of fluid media
1-Alkaline peptone water
Is an enrichment media used for the cultivation of vibrio spp. From feces and other infected materiales
Ph=8.4
This help vibrio spp. For growth
Samples that contain vibrio spp. Must be inoculated on alkaline peptone water befor inoculation on TCBs
2-Christensen,s urea broth
Used for detection of urease activity of proteus spp.
3-Cooked meat medium
An enrichment media used for cultivating of anaerobes bacteria as clostridia sp
4-glucose phosphate peptone water
For culturing of entrerobacteriaceae in the Voges-proskauer test
5-Koser citrate medium
Used for differentiating E,Coli from Enterobacter.aerogenosa based on citrate utilizatione
Koser found the sodium salt of citric acid is used as a source of carbon by E.aerogenosa and not by E.COLI
6- Litmus milk medium
Used for detecting litmus milk reduction in identifying entrerococci and some clostridium spp.
In this medium
Lactose(milk sugar)
Litmus(pH indicator)
Caseine(milk protein)
Process that occure by action of bacteria in this media
a- fermentation of lactose
if lactose fermented by bacteria the solution turn into pink colour
b- form gas
if gas produced during fermentation, you can see bubbles in the milk
c-reduce litmus
if lactose not fermented ,the solution become alkaline and blue colour
d-form clot
Casein protein may be digested,this will coagulate milk to form asolid solution
e-peptonization
casein protein metabolized into individual amino acid
7-nitrate broth
Used for detecting nitrate reduction in identifying mycobacterium spp. And gram negative bacteria
8-peptone water sugar
carbohydrate testing medium for differentiating enteric pathogen
9-Selenite F broth
Is used as enrichment medium for the isolation of salmonella spp. From feces , urine and water
10-Thioglycolate broth
this medium used to determine the degree to which microorganism can grow with or without oxygen
this medium contain sodium thioglycolate,which react with molecular oxygen kepping free oxygen level low
Tube 1: Obligate Anaerobe -- note the absence of growth in the top portion of the broth where oxygen is present.
Tube 2: Obligate Aerobe -- note the growth is only in the top portion of the tube where oxygen is present.
Tube 3: Aerotolerant -- note the uniform growth from top to bottom.
Tube 4: Facultative -- note the uneven distribution of growth from top to bottom (more growth at the top).
Tube 5: Obligate Aerobe -- note the growth is only in the top portion of the tube where oxygen is present.
Todd Hewitt broth -11
Used for culturing beta-hemolytic streptococci
12-Trypton water
used for detection ofE.COLI in food and water samples based on indole production
ِِ
اسعدتم صباحا
اخوكم ابو النور
تعليق