تويت
1- What is the principle underlying the Ouchterlony gel diffusion method/test. Are there any clinical laboratory tests in which this principle is applicable or used ? Name one
such test and describe its outcomes ?
2. You purchased chicken-mince meat from the market but when you opened the packet at home, you found that the color was a bit too red due to blood oozing out of the chicken mince meat to believe that the chicken mince was not mixed with red meat from sheep. How would you prove using the Ouchterlony gel diffusion method that there was contamination of chicken mince-mince meat with sheep meat. Assume that you can purchase any immunological reagent that is required by you
for use in these techniques/methods to prove that the chickenmince meat was indeed contaminated. Support your answer using a diagrammatic sketch or sketches.
3. As the Principal Medical Scientist in the local hospital, you ordered goat anti-human Fc[y] antibody. What positive andnegative control Ouchterlony gel diffusion tests would you
need to carry out to assure that the antibody indeed is anti-Fc[y] and not against any other immunoglobulin heavy or light
chains? .
such test and describe its outcomes ?
2. You purchased chicken-mince meat from the market but when you opened the packet at home, you found that the color was a bit too red due to blood oozing out of the chicken mince meat to believe that the chicken mince was not mixed with red meat from sheep. How would you prove using the Ouchterlony gel diffusion method that there was contamination of chicken mince-mince meat with sheep meat. Assume that you can purchase any immunological reagent that is required by you
for use in these techniques/methods to prove that the chickenmince meat was indeed contaminated. Support your answer using a diagrammatic sketch or sketches.
3. As the Principal Medical Scientist in the local hospital, you ordered goat anti-human Fc[y] antibody. What positive andnegative control Ouchterlony gel diffusion tests would you
need to carry out to assure that the antibody indeed is anti-Fc[y] and not against any other immunoglobulin heavy or light
chains? .
k i