a method of detecting antigens or antibodies on a cell surface using antibody- or antigen-coated particles, which cause erythrocytes to form a rosette pattern.
As example, a mixed-immunoglobulin rosette technique has been developed for the detection of antibodies to feline oncornavirus-associated cell membrane antigens. Lymphoblastoid cells infected with feline leukemia virus were incubated with test sera and then fixed in paraformaldehyde. They were then exposed to a rabbit anti-cat immunoglobulin G serum. Antigen-antibody reactions were detected by mixing the cells with sheep erythrocytes sensitized with cat anti-sheep red blood cell serum; the presence of cat antibody on the lymphoid cells was registered by the formation of sheep red blood cell rosettes around them. The method was shown to be at least 10 times more sensitive than indirect immunofluorescence
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