بس بتمني لو اي واحد امتحن الهيئة قريب في المايكرو ينزل لينا الاسئلة وربنا يجعلها في ميزان حسناتكم انشاء اللة ودعواتكم
- Acid Fast Bacteria
a- Salmonella
b- Shigella
c- M. Tuberculosis
d- E – Coli
192- Spherical or avoid cells occurring in chains
a- Staphylococci
b- Streptococci
c- Lactobacillus
d- Spiro chaeta

193- ……………… carries the genetic information
a- the envelope
b- the capsid
c- the nucleic acid
d- the prion

194- …………………… may be seen under light microscope
a- rota virus
b- influenza virus
c- herps virus
d- pox virus

195- viruses may be:
a- monomorphic
b- pleomorphic
c- dimorphic
d- all of the above

196- viral capside is formed of:
a- protein
b- glycogen
c- lipoprotein bilayer
d- glycoprotein

197- class III in Baltimor classification is:
a- double stranded DNA viruses
b- single stranded DNA viruses
c- double stranded RNA viruses
d- single stranded RNA viruses

198- Hierarchial virus classification system use the following characters except:
a- nature of nucleic acid
b- capside symmetry
c- diameter of viron & capside
d- virus molecular weight
199- in viral replication which is true:
a- penetration is the 1st step
b- assembly is the last step
c- relaease is the last step
d- all of the above

200- viron:
a- may be extracellular phase of virus
b- may be intracellular phase of virus
c- can grow and replicate
d- means “ virus – like “

201- pleomorphic viruses means :
a- virus which have constant shape
b- virus that may appear in 2 forms
c- virus that have not a constant morphology
d- virus that have spherical shape

202- vapor of gold is used in :
a- shadow casting technique
b- negative staining technique
c- positive staining technique
d- non of the above

203- direct diagnosis of virus :
a- ELISA Antibody
b- CFT
c- IFT
d- PCR virus

204- all of the following are required in cell culture except:
a- neutral PH
b- presence of buffer salts
c- presence of antibiotics
d- incubation at 20 C (( 30-37 C))

205- all of the following are diagnostic molecular biological technique except:
a- PCR
b- ELISA
c- nucleic acid hyperdization
d- DNA finger printing
206- PCR require all of the following except:
a- extracted DNA template
b- 2 specific primers
c- reation buffer
d- RNA polymerase

207- bacteriophage is :
a- virus that can be killed by antibiotic
b- virus that act like a bacteria
c- bacteria that act like a virus
d- virus that infect bacteria

208- all of the following viruses are transmitted by blood except:
a- HIV
b- HBV
c- HCV
d- herps virus

209-all of the following are RNA viruses except:
a- corona viridase
b- reoviridase
c- picorona viridase
d- pox viridase

210- penetration of naked virus is by :
a- fusion
b- endocytosis
c- translocation
d- all of the above

211- amniotic cavity inoculation is one type of virus culture in:
a- tissue wall
b- lab animals
c- embryonated egg
d- non of the above

212- …………….. is an invitro method amplification of a short sequence of target DNA
a- PCR
b- hyberdization
c- finger printing
d- all of the above

213- nucleic acid hyberdization means:
a- probe anneling or binding with it’s complementary
segment of NA
b- fragmentation of nucleic acid
c- amplification of nucleic acid
d- non of the above

214- DNA hyberdization is performed by:
a- primers
b- DNA labeled probe
c- restriction endonuclease
d- non of the above

215-PCR starts with :
a- annealing
b- denaturation of DNA
c-extension of primers
d- non of the above

216- ……………….. is a piece of DNA fragment of a particular gene that can bind specially with it’s complementary piece of DNA:
a- codon
b- probe
c- LCR
d- code

217- how many primers are used in PCR :
a- non
b- one
c- two
d- three

304-Lowenstein-Jensen media is used for the isolation for:
a- neisseria gonorrhea
b- mycobacterium tuberculosis
c- haemophilus influenza
d- staphylococcus aureus



305- Hekton-Enteric agar is the selective media for:
a- salmonella
b- streptococcus
c- staphylococcus
d- all of the above

306- the selective media for isolation of fungi is:
a- S-S agar
b- XLD agar
c- sabouraud glucose agar
d- Hekton-Enteric agar

307- gram positive rods with Chinese letter appearance:
a- mycobacterium tuberculosis
b- corynebacteria diphtheria
c- clostridium tetani
d- staphylococcus pneumonia

308- E lek test is done to diagnose :
a- streptococcus
b- staphylococcus aureus
c- clostridium tetani
d- corynebacteria diphtheria

309- all are lactose fermenter except:
a- E-COLI
b- proteus
c- klebsiella
d- enterobacter

310- produce pale colonies on MacConkey’s agar and have tendency to swarm on blood agar:
a- salmonella
b- shigella
c- klebsiella
d- proteus





311- lactose frementer gram negative bacilli with mucoid growth:
a- salmonella
b- shigella
c- klebsiella
d- proteus

312-non lactose fermenter gram negative bacilli produce H2S :
a- salmonella
b- shigella
c- klebsiella
d- proteus

313- Widal test is done for diagnosis of :
a- salmonella
b- shigella
c- E-coli
d- klebsiella

314- the causative agent of enteric fever:
a- salmonella
b- shigella
c- klebsiella
d- proteus

315- the most common cause of urinary tract infection :
a- E-coli
b- salmonella
c- shigella
d- streptococcus

316- an important cause of diarrhea in infant:
a- staphylococcus
b- E-coli
c- salmonella
d- shigella

317- the most common causative agent for peptic ulcer :
a- campylobacter
b- H-pylori
c- V-cholera
d- all of the above
318- TCBS is the selective media for isolation of:
a- H-pylori
b- V-cholera
c- E-coli
d- H influenza

319- gram negative bacilli strict aerobes grows on simple media producing a characteristic greenish pigment:
a- campylobacter
b- pseudomonas
c- pasterulla
d- bordetella

320- the bacteria which cause scarlet fever:
a- staphylococcus
b- streptococcus
c- salmonella
d- shigella

321- antistreptolysin O titer (ASO) is done for the diagnosis of:
a- group A streptococcus
b- group B streptococcus
c- staphylococcus aureus
d- staphylococcus albus

323- Loffler’s serum is used for isolation of:
a- anthrax
b- clostridium
c- diphtheria
d- T.B

324- gas gangrene is caused by:
a- clostridium tetani
b- clostridium botulinum
c- clostridium welchii
d- non of the above (( clostridium perfinges ))

325- Bacillary dysentery is caused by:
a- salmonella
b- shigella
c- cholera
d- all of the above

326- the cause of plague:
a- Y-enterocolitica
b- Y-pestis
c- Y pseudotuberculsois
d- non of the above

327- treponema palladium is the cause of :
a- T.B
b- gonorrhea
c- syphilis
d- AIDS

328- the venereal disease research laboratory test (VDRL) is done for diagnosis of:
a- T.B
b- gonorrhea
c- syphilis
d- AIDS

329- Trachoma is caused by:
a- mycoplasma
b- chlamydiae
c-richettsia
d- mycobacteria

391- M-tuberculsis bacilli stain with :
a- gram stain
b- Zheil Nelson stain
c- Gimesa stain
d- all of the above

392- AIDS is transmitted through :
a- food
b- blood
c- semen
d- (b) & (c)
393- bacteria which cause syphilis:
a- Neisseria gonorrhea
b- Viencent angina
c- Treponema palladium
d- Yersinia pestis

394- to diagnose syphilis:
a- RPR
b- VDRL
c- Wasserman
d- all of the above

395- streptococci secret:
a- streptolysin O
b- streptolysin S
c- streptokinase
d- all of the above

396- disease caused by streptococci:
a- scarlet fever
b- purperal sepsis
c- rheumatic fever
d- all of the above

397- staphylococci secrets:
a- coagulase enzyme
b- fibrinolysin
c- hyaluronidase
d- all of the above

398- gram positive bacilli:
a- Klebsilla
b- Salmonella
c- Proteus
d- C-diphtheria

399- meningeococcal meningitis is transmitted by :
a- food
b- droplet
c- touch
d- all of the above

400- dark field microscopy is used to diagnose :
a- T.B.
b- syphilis
c- gonorrhea
d- AIDS

401- it cause food poisining with flacid paralysis:
a- clostridium tetani
b- clostridium welchii
c- clostridium botulinium
d- all of the above

402- the infective stage of plasmodium vivax :
a-merozoites
b- sporozoites
c- schizont
d- trophozoite

403- Pirenella conica snail is the intermediate host of:
a- schistosoma haematobium
b- fasciola hiptica
c- heterphyes heterophyes
d- diphyllobothrium latum

404- Bulinus truncates snail is the intermediate host of :
a- fasciola hepatica
b- fasciola gigantica
c- schistosoma haematobium
d- schistosoma mansonii

405- to isolate meningiococci we have to culture the sample on:
a- Bordet Gengou
b- modified Thayer martin media
c- Lowenstein Jensen media
d- all of the above

406- to isolate fungi :
a- Brain-Heart infusion media
b- tissue culture
c- Lowenstein –Jensen media
d- chocolate agar

407- to isolate H- influenza:
a- blood agar
b- chocolate agar
c- mac Conkey media
d- all of the above

408- the bacteria which cause pseudomembrainous conjunctivitis :
a- N.gonorrhea
b- C. diphtheria
c- staphylococcus
d- Chlamydia

409- the best sample to diagnose meningitis :
a- blood
b- sputum
c- CSF
d- urine

410- used to stain Chlamydia
a- gram stain
b- giemsa stain
c- wright stain
d- all of the above
411-……….. is used as transport medium for sample in which cholera is suspected
a- Cary-Blair media
b- Stuart media
c- Alkaline peptone water
d- glycerol

412- the color of XLD medium:
a- green
b- red
c- yellow
d- blue

413- CIN medium is used to isolate:
a- E.coli
b- Vibrio cholera
c- yersinia
d- salmonella

414- to make wet mount preparation:
a- 10 % KOH
b- 10 % Na OH
c- 10 % Na CO3
d- 10% Na Cl

415- we do wet mount preparation for vaginal smear To diagnose:
a- T.vaginalis
b- N.gonorrhea
c- streptococci
d- staphylococci

416- to isolate viruses:
a- Loeffler media
b- tissue culture
c- Bordet –Gengou media
d- Brain- Heart infusion

417- to diagnose whooping cough :
a- Bordet –Gengou media
b- Lowenstein –Jensen media
c- modified Thayer martin media
d- New York city agar

418- to diagnose systemic infection we do :
a- urine culture
b- CSF culture
c- blood culture
d- sputum culture

419- we give no growth for blood culture after:
a- 1 week
b- 8 weeks
c- 6 weeks
d- 3 weeks





420- to dissolve mucous in sputum sample :
a- 10% NaOH
b- 30 % NaOH
c- 10 % KOH
d- 10% NaCl

421- mutualism means:
a- one partener benefits , other unaffected
b- both partner benefit
c- one partner benefit , other damaged
d- living together

422- Commensalisms means:
a- living together
b- one partner benefit , other damaged
c- both partner benefit
d- one partner benefits , other unaffected

423- Balantidium coli moves by:
a- flagella
b- cilia
d- pseudopod
d- all of the above

424- Mouth inhabitant:
a- Trichomonas hominis
b- Trichomonas tenax
c- Trichomonas vaginalis
d- giardia lamblia

425- Transmitted by sexual intercourse:
a- toxoplasma
b- giardia lamblia
c- Trichomonas vaginalis
d- all of the above

465- citrate utilization test is done to assist identification of:
a- gram +ve bacteria
b- gram –ve bacteria
c- entrobacteria
d- enterococcus

466- the Kovac's reagent used in the following biochemical tests:
a- catalase
b- coagulase
c- indole
d- methyl red

467- positive results for H2S production appear as …. Colour:
a- black
c- yellow
c- red
d- green

468-………. Give positive coagulase test:
a- streptococci
b- staphylococcus aureus
c- staphylococcus saprophyticus
d- Escherichia coli

469- methyl red test is performed with:
a- Erlich reagent
b- Kovac's reagent
c- Voges proskaur
d- non of the above

470- ………… give positive result with urease test:
a- salmonella
b- shigella
c- Y. enterocolitica
d- all of the above

471-…………. test is used to differentiate between bacteroides & brucella:
a- indole
b- methyl red
c- H2S production
d- nitrate reduction

472- DNAase test is positive with:
a- streptococcus pneumonia
b- E.coli
c- staphylococcus aureus
d- staphylococcus epidermis

473- ………… solution used in the gram stain technique acts as a mordant:
a- crystal violet
b- safranine
c- iodine
d- alcohol

474- the counter stain in Ziehl- Neelson stain is :
a- malachite green
b- methylene blue
c- iodine
d- (a) & (b)

475- bile solubility test is positive with :
a- streptococcus viridans
b- streptococcus pneumonia
c- streptococcus agalectiae
d- streptococcus pyrogenes
During replication of DNA, copying errors may occur and this is called
a- Conjugation
b- Transduction
c- Transformation
d- Mutation

132- Obligatory Anaerobic bacteria
a- grow only in presence of oxygen
b- grow only in absence of oxygen
c- grow either in presence or absence of oxygen
d- grow in presence of oxygen traces and 5 – 10% CO2

133- Neutrophilic bacteria grow well at
a- pH 8.5 – 9.0
b- pH 7.2 – 7.4
c- pH 5.0 – 5.5
d- pH 2.5 – 3.0

134- The rate of cell death increase and bacterial growth stopped, this is
a- Adaptation phase
b- Exponential phase .
c- Stationary phase.
d- Decline Phase

135- Beta-hemolytic
a- Cause complete hemolysis of RBC’s
b- Cause chemical change of Hemoglobin in RBC’s
c- Do not cause hemolysis
d- None of them





146- Mesophilic bacteria grow at:
a- 37°C
b- 14°C
c- 60°C
d- 120°C

137- Circulation of Bacteria and its toxins in blood
a- Pyaemia
b- Toxemia
c.- Bacteremia - without toxins
d- Septicemia

138- Disinfections that applied on living or injured tissues:
a- Sterilization
b- Antiseptic
c- Sanitation
d- Decontamination

139- Hot air oven is used for sterilization of
a- Glass
b- Rubber Gloves
c.- Plastic Syringes
d- Catheters

140- Disinfectant for superficial fungal infection
a- Phenol
b- Potassium permanganate
c- Chlorine
d- Hypochlorite compounds

141- Rifampin
a- inhibit cell wall synthesis
b- inhibit protein synthesis
c- inhibit folic acid pathway
d- inhibit mRNA synthesis

142- Transacetylase inactivate
a- aminoglycosides
b- chloramphenicol
c- penicillin
d- cephalosporins

143- The color of gram positive bacteria is
a- Yellow
b- Black.
c- Pink
d- Violet

144- Selective media for fungi
a- blood agar
b- Mac Conkey agar
c- Nutrient agar
d- Sabourand’s dextrose agar

145- Histoplasma is a :
a- Systemic mycosis
b- Sub – Cutaneous mycosis
c- Cutaneous mycosis
d- Superficial mycosis

146- All are asexual spores produced by mould except
a- Conidio – spores
b- Sporangio – spores
c- Endospores
d- Arthro – spores

147- They reproduce only by Asexual reproduction
a- Blastomycosis
b- Deutromycosis
c- Ascomycetes
d- Zygomycetes

148- The functions of cell wall is all of the following except:
a- Giving the shape to the bacteria
b- Carrying somatic antigen
c-Selective permeability& transport of solutes = plasma membrane
d- Protect the bacteria from plasmolysis

149- Atrichous Bacteria are:
a- Bacteria contain one flagellum
b- Bacteria contain 2 flagella
c- Bacteria without flagella
d- Bacteria with a tuft of flagella

150-__________ are essential for host cell attachment:
a- Flagella
b- Fimbria
c- Spores
d- Capsules

151- Irregular clusters of spherical cells:
a- Streptococcus
b- Staphylococcus
c- Lactobacillus
d- Escherichia

152- Clostridium Botulinum is:
a- Obligatory Aerobic Bacteria
b- Facultative Anaerobic Bacteria
c- Obligatory Anaerobic Bacteria
d- Micro- aerophilic Bacteria

153- Basophilic Bacteria grow well at:
a- pH 8.5 – 9.0
b- pH 7.2 – 7.4
c- pH 5.0 – 5.5
d- None of the above

154- Cells are divided at high & constant rate:
a- Decline Phase
b- Stationary Phase
c- Log Exponential Phase
d- Adaptation Phase

155- The dominant bacterial species in dental plaque are:
a- Coagulase Negative Staphylococci
b- Lactobacillus
c- Bacteroides
d- Streptococcus Sanguis

156- The spread of Pyogenic Bacteria in blood stream to different organs & produce multiple abscess is:
a- Septicemia
b- Bacteremia
c-Toxemia
d- Pyaemia
157- Inhibit the growth of micro organisms
a- Bacteriostatic
b- Bactericidal
c- Fungicidal
d- Germicidal

158- To sterilize fluid damaged by heat:
a- Gaseous Sterilization
b- Heat Sterilization
c- Filtration
d- Ionizing Radiation

159- For water disinfection we use:
a- Hydrogen peroxide
b- Formaldehyde
c- Chlorine
d- Hypochlorite compounds

160- Mechanism of action of penicillin:
a- Block peptidoglycan synthesis
b- Inhibit peptidyglycan cross – linking
c- inhibit folic acid pathway
d- inhibit protein synthesis

161- Sulfonamides:
a- inhibit cell wall synthesis
b- inhibit protein synthesis
c- inhibit DNA synthesis
d- inhibit folic acid pathway

162- Acetylase inactivates:
a- B – Lactam antibiotics
b- Aminoglycosides
c- Cloramphenicol
d- All of the above

163- Ringworm disease is caused by
a- Zygomycetes
b- Ascomycetes
c- Blastomycosis
d- None of the above

164- For wet – mount technique we add:
a- NaoH
b- K oH
c- H2 O2
d- All of the above

165- They are transmitted by arthropods
a- Chlamydia
b- Spirochetes
c- Mycoplasma
d- All of the above

166- In the past they were listed as large viruses
a- Richettsia
b- Mycoplasma
c- Chlamydia
d- None of the above

167- Bacteria multiply by:
a- Replication cycle
b- Simple binary fission
c- Sexual reproduction
d- All of the above

168- It protects bacteria from antibiotics
a- Capsule
b- Cell membrane
c- Flagella
d- Fimbria

169- Vibro cholera is:
a- Mono –trichous bacteria
b- Atrichous bacteria
c- Lopho-trichous bacteria
d- Peri-trichous bacteria

170- Short rods, motile by peritrichous flagella
a- Spirochaeta
b- Lactobacillus
c- Escherichia coli
d- Vibrio
171- To take up soluble DNA fragments derived from other, closely related species is:
a. Mutation
b. Transformation
c. Transduction
d. Conjugation

172- Tuberculosis are
a- micro-airophilic
b- Facultative anaerobic
c- Obligatory anaerobic
d- Obligatory aerobic

173- According to pH, Lactobacillus is
a- Neutrophlic bacteria
b- Acidophilic bacteria
c- Basophilic bacteria
d- None of the above

174- Bacteria without cell Wall
a- Chlamydia
b- Rickettsia
c- Mycoplasma
d.- Spirochetes

175- Brucella Melitensis is
a- Obligatory aerobic bacteria
b- Obligatory anaerobic bacteria
c- Facultative anaerobic bacteria
d- Micro-aerophilic bacteria

176- Pseudomonas aeroginosa is
a- Peri-trichous bacteria
b- Lopho-trichous bacteria
c- Amphi-trichous bacteria
d- Monotrichous bacteria

177- Genetic information of bacteria is carried on
a- Messenger RNA
b- Transfer RN|A
c- Tran****** RNA
d- Double – Stranded DNA
178- Thermophilic bacteria grow at
a- 60 – 80 °C
b- 0 - 20°C
c-. 37°C
d- 100 - 120°C

179- Acidophilic bacteria grow at
a- pH 7.2 – 7.4
b- pH 5.0 – 5.5
c- pH 8.5 – 9.0
d- None of the above

180- Mycoplasma is
a- Neutrophilic bacteria
b- Acidophilic bacteria
c- Basophilic bacteria
d- All of the above

181- It is the adaptation of bacteria to the fresh medium
a- Lag phase
b- Decline phase
c- Logarithmic Phase
d- Stationary phase

182- Bacteria which do not cause hemolysis is
a- Beta-Hemolytic
b- Alpha-Hemolytic
c- Gama Hemolytic
d- None of the above

183- Normal flora of Lower intestine are all of the following except:
a- Staphylococci
b- Diphtheroids
c- Shigella
d- Lactobacillus

184- Opportunistic pathogens are all of the following except:
a- Cause a disease when the host defense are suppressed.
b- Are normal flora of healthy body
c- Are greatly harmful
d- Do not invade the body or tissue.
185- For disinfection of mattresses :
a- Hot air oven
b- Autoclave
c- Ethylene Oxide
d- Hydrogen Peroxide

186- Pyschrophilic bacteria grow at:
a- 10٠°C
b- 6٠°C
c- 14°C
d- 37°C

187- Bacteria which contain chlorophyll
a- Heterotrophic bacteria
b- Autotrophic bacteria
c- Photosynthetic bacteria
d- All of the above

188- Tricophyton is one of
a- Yeast
b- Moulds
c- Dermatophyte
d- Dimorphic Fungi

189- Plastomyces is one of
a- Dermatophytes
b- Dimorphic Fungi
c- Yeast
d- Moulds

190- Color of gram negative bacteria is
a- Violet
b- Green
c- Red
d- Black


BACTERIOLOGY
118- All are Prokaryotic cells except:
a- Fungi
b- Bacteria
c - Chlamydia
d- Mycoplasma

119- Viruses:
a- Contain only DNA or RNA
b - They Contain ribosome
c- Did not affected by antibiotics
d- a+c

120- All of these are essential structure except:
a- Nuclear body
b- Spores
c- Cell wall
d- Plasma Membrane

121- ________ is giving the shape to the bacteria
a.- Cytoplasmic Membrane
b- Capsule
c- Cell Wall
d- All of the above

122- One of its functions is selective permeability
a- Cell wall
b- Plasma membrane
c- Capsule
d- Spores

123- They are responsible for Haemagglutination Phenomenon
a- Flagella
b- Fimbria
c- Capsule
d- Cell wall





124- Clostridium Tetani is:
a- Atrichous bacteria
b- Mono-trichous bacteria
c- Amphi-trichous bacteria
d- Peri-trichous bacteria

125- Short curved or straight rods, motile by single polar flagellum
a- spirochaeta
b- Vibrio
c- Escherichia
d- Lactobacillus

126- Small gram negative cocci, occur in pairs
a- staphylococcus
b- streptococcus
c- neisseria
d- non of the above

127- Transfer of genetic information from one bacterium to another by
bacteriophages is:
a. Transformation
b. Tansduction
c. Conjugation
d. Mutation

128- Salmonella are:
a- Obligatory Aerobic bacteria
b.- Obligatory Anaerobic bacteria
c- Facultative Anaerobic bacteria
d- Micro-aerophilic bacteria

129- According to pH, vibrio cholera is
a- Osmophilic bacteria
b- Basophilic bacteria
c- Acidophilic bacteria
d- Neutrophilic bacteria




130- Staphylococci are:
a- Atrichous bacteria
b- Mono-trichous bacteria
c- Amphi-trichous bacteria
d- Peri-trichous bacteria