تويت
Microtome typesObjectives Definition and principle of microtomy
Type of microtome and their specific purposes
type of microtome knife
Sharpening
Factor effecting section quality
Definitionis a sectioning instrument that allows for cutting thin slices 3 – 5 micron of material, known as sections.
Microtome's are an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electronic .
Rocking microtome Small lightweight
Suitable for class-work and teaching
Knife fixed and block holder is move
Producing curved section
Rotary microtmeThe most popular ,used mainly for paraffin wax embedded material
Production serial section (ribbon)
Knife fixed and block move against the knife
Heavy microtome
Used in cryostat
Sliding microtomeUsed mainly for cutting of cellulose nitrate embedding tissue
Knife moved across of objective surface
Use for cutting of whole organ Base sledge microtomeFixed knife and travel objective
Used cellulose nitrate embedded tissue
Suitable for cutting bone tissue
CO2 freezing microtomeUsed for fixed and unfixed tissue
Liquid / gas Co2 (-66°c)
Not possible to prepare ribbon
CryostatFreezing option with liquid N2 (-166°c)
Used rotary microtome as cutting device
Used for rapid diagnosis :
Urgent biopsy
Lipid demonstration
Enzyme histochemistry
Immunohistochemistry
Infectious hazard
Ultra microtome Use for very thin sections Which is exam under electronic microscope
The thickness of tissue
(0.005 – 0.1 micron)
The knife is usually glass or dimon .
Sharpening Manual or mechanical
The sharpening step include two stage
Stage -1 Honing : renewing bevel and restore the edge to straight line by using Belgian stone
Stage-2 stropping (polishing ) by using leather
Factor effecting section qualityFixation (well fixed and variation b/w fixative)
Processing
Embedding (well oriented)
Microtome type
Knife (Sharpe and clean)
Cutting technique
Type of microtome and their specific purposes
type of microtome knife
Sharpening
Factor effecting section quality
Definitionis a sectioning instrument that allows for cutting thin slices 3 – 5 micron of material, known as sections.
Microtome's are an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electronic .
Rocking microtome Small lightweight
Suitable for class-work and teaching
Knife fixed and block holder is move
Producing curved section
Rotary microtmeThe most popular ,used mainly for paraffin wax embedded material
Production serial section (ribbon)
Knife fixed and block move against the knife
Heavy microtome
Used in cryostat
Sliding microtomeUsed mainly for cutting of cellulose nitrate embedding tissue
Knife moved across of objective surface
Use for cutting of whole organ Base sledge microtomeFixed knife and travel objective
Used cellulose nitrate embedded tissue
Suitable for cutting bone tissue
CO2 freezing microtomeUsed for fixed and unfixed tissue
Liquid / gas Co2 (-66°c)
Not possible to prepare ribbon
CryostatFreezing option with liquid N2 (-166°c)
Used rotary microtome as cutting device
Used for rapid diagnosis :
Urgent biopsy
Lipid demonstration
Enzyme histochemistry
Immunohistochemistry
Infectious hazard
Ultra microtome Use for very thin sections Which is exam under electronic microscope
The thickness of tissue
(0.005 – 0.1 micron)
The knife is usually glass or dimon .
Sharpening Manual or mechanical
The sharpening step include two stage
Stage -1 Honing : renewing bevel and restore the edge to straight line by using Belgian stone
Stage-2 stropping (polishing ) by using leather
Factor effecting section qualityFixation (well fixed and variation b/w fixative)
Processing
Embedding (well oriented)
Microtome type
Knife (Sharpe and clean)
Cutting technique