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موضوع مهم؟!!! وخطير جدا

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  • موضوع مهم؟!!! وخطير جدا

    السلام عليكم اخواني واخواتي
    سؤالي مهم جدا


    معمل الhistology
    ايش هي الطرق الي نعقم بيها المحاليل بشكل عام

    يعني مثلا السوائل او بيئه سائله

    محاليل التثبيت ؟
    يعني اي شي سائل بشكل عام كيف نعقمه لان صعب نعقمه باتوكلايف

    وغير كدا ايش الفرق بين تعقيم الشي وتطهيره disinfection

  • #2
    السلام عليكم
    عل ما اظن انك ما تحتاج لتعقيم السوائل في histo لأن ما تهمك لو كان فيها بكتريا لو لا.
    وغالبا السوائل في هذا القسم (الفورملدهايد مثلا) تعتبر disinfectants يعني بعد ما يوضع فيها اي شيء كل اللي فيه يموت. وهذا مختصر ان شاء الله يفيدك شوي


    Sterilisation is bactericidal; that is, sterilisation kills all living organisms in or on an object or substance or within a biological matrix. This is useful as it removes all pathogenic or spoilage microorganisms. However, sterilisation is usually produced by autoclaving with the use of high temperatures and pressures. These temperatures and pressures can preclude the sterilisation of heat sensitive substances (us for example!) and objects that are too large to fit into autoclaves. Therefore, chemical sterilisation by disinfection or antisepsis at room temperature is often necessary. Disinfectants are described as antimicrobial agents used on inanimate objects such as instruments
    (medical or dental), plastics or structural surfaces (kitchen surfaces, toilets, washbasins etc.). If a disinfectant frees an object of all infectious agents, then sterilisation has occurred. The term antiseptic is applied to antimicrobial agents that are used on living tissue such as skin and throat mucosa. The majority of disinfectants are too toxic to use on humans as they are not selective in their antimicrobial action.

    Are disinfectants and antiseptics sterilising agents? Disinfectants and antiseptics do not always sterilise because these compounds usually do not kill all fungal and bacterial spores and vegetative bacteria and fungi. Organic compounds can interfere with their action reducing the efficiency of the antimicrobial agent.


    Here are a few of the many types of disinfectants:

    Phenolic compounds
    1. Concentrated cresol ( for protecting outdoor wood)
    2. Diluted and saponified (Lysol, used in microbiology to disinfect contaminated benches, glassware))

    Alcohols (up to 70%)
    1. Ethyl ( Hospitals)
    2. Isopropyl ( Good for work surfaces)

    Synthetic detergents = QAC (quaternary ammonium compound)
    1. Roccal
    2. Zephiran

    Sterilising gases
    1. Formaldehyde
    2. Ethylene oxide mixtures (Carboxide, Cryoxide)

    No single disinfectant is ideal. Each has its advantages and disadvantages. For example, phenols sterilise well but are corrosive and toxic. Detergents and 70% alcohol have some microbiocidal effect but are not sporicidal and dry out skin surfaces.

    وشكرا
    :sm188:It is nice to be important but, it is important to be nice

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