لماذا عينة هرمون ACTH تكون في EDTA Tube وتكون في ثلج ice
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لماذا عينة هرمون ACTH تكون في EDTA Tube وتكون في ثلج ice؟
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* ACTH is unstable and samples must be collected into EDTA with the plasma being separated and frozen within 30 min of collection.
* Plasma [ACTH] should be measured on blood specimens collected both in the morning and the evening (e.g. at 08:00 AM and 22:00 PM).
(1) If ACTH is undetectable, this is diagnostic of adrenal tumour, and should be confirmed by an abdominal CT scan to detect an adrenal mass.
(2) If the patient has Cushing's disease (pituitary-dependent Cushing's syndrome), ACTH will be present in plasma in normal or increased amounts, particularly in the evening specimen.
(3) Results for plasma [ACTH] overlap considerably for patients with Cushing's disease and ectopic ACTH secretion. However, very high plasma [ACTH] points to an ectopic origin.
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