Coombs Test procedure
1. Take 4 tube , 3 for Indirect and 1 for Direct coombs test
2. do ABO blood group and Rh ( D) and Control.
3. Add 1 drop of cell 1 , cell2 , cell3 in all indirect tube only .
4. add 3 drop of serum suspension of all tubes ( Direct and indirect ) but Direct 2drops
5. add 2drop of Low Ionic strength solution to become the reaction between Ag+Ab
6. incubation 15 min, in water bath
7. make washing 4 times for Direct and in direct tube for coombs test
8. add 2drops of Coombs reagent (anti anti body) , centrifuge 15 sec
9. read in microscope
If Indirect coombs test _ve its mean No allow Ab .
If Direct Coombs test _ve No Auto Ab.
10. Add 1drop of coombs control. ( to confirm for the work ) , Centrifuge for 15 sec. مع تحيات ابو سيزار مشكورين
1. Take 4 tube , 3 for Indirect and 1 for Direct coombs test
2. do ABO blood group and Rh ( D) and Control.
3. Add 1 drop of cell 1 , cell2 , cell3 in all indirect tube only .
4. add 3 drop of serum suspension of all tubes ( Direct and indirect ) but Direct 2drops
5. add 2drop of Low Ionic strength solution to become the reaction between Ag+Ab
6. incubation 15 min, in water bath
7. make washing 4 times for Direct and in direct tube for coombs test
8. add 2drops of Coombs reagent (anti anti body) , centrifuge 15 sec
9. read in microscope
If Indirect coombs test _ve its mean No allow Ab .
If Direct Coombs test _ve No Auto Ab.
10. Add 1drop of coombs control. ( to confirm for the work ) , Centrifuge for 15 sec. مع تحيات ابو سيزار مشكورين
تعليق