هذه نبذة عن الصبغة
The Ziehl-Neelsen stain, also known as the acid-fast stain, was first described by two German doctors; Franz Ziehl (1859 to 1926), a bacteriologist and Friedrich Neelsen (1854 to 1894), a pathologist. It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. Mycobacterium tuberculosis is the most important of this group, as it is responsible for the disease called tuberculosis (TB). It is helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain. It can also be used to stain few other bacteria like Nocardia. The reagents used are Ziehl-Neelsen carbolfuchsin, acid alcohol and methylene blue.
السلام عليكم
بالنسبة للزييل نيلسن هو عبارة عن صبغة للبكتيريا العصوية المسسبة لمرض السل
و مبدأ العمل له
هو أن هذا النوع من البكتيريا يحتوي جدارها على الزيوت فيمنع امتصاص الحوامض
لذلك نقوم بعملية التسخين حتى تتكسر هذه الدهون و تاخذ البكتيريا الصبغة
هناك عدة صبغات منها الفلوريسنت و الكولد زييل نيلسن
اي سؤال اخر انا حاضرة
اتمنى أكون أفدتك
STEP 2:
Flood the entire slide with Carbol Fuchsin
Ensure enough stain is added to keep the slides covered throughout the entire staining step.
STEP 3:
Using a Bunsen burner, heat the slides slowly until they are steaming. Maintain steaming for 5 minutes by using low or intermittent heat (i.e. by occasionally passing the flame from the Bunsen burner over the slides)
Caution: Using too much flame or heat can cause the slide to break.
STEP 4:
Rinse the slide with water.
STEP 5:
Flood the slide with 3% acid-alcohol and allow to decolorize for 5 minutes.
Throughout the 5 minutes, continue to flood the slides with 3% acid-alcohol until the slides are clear of stain visible to the naked eye.
To the right are examples of slides insufficiently and sufficiently flooded with 3% acid-alcohol.
STEP 6:
Rinse the slide thoroughly with water and then drain any excess from the slides.
STEP 7:
Flood the slide with the counterstain, Methylene Blue. Keep the counterstain on the slides for 1 minute.
STEP 8:
Rinse the slide thoroughly with water.
If all steps are performed correctly you should have a slide that looks like this.
ACID-FAST BACTERIA - ZIEHL-NEELSEN STAIN (AFB)
PURPOSE: Used in the demonstration of acid-fast bacteria belonging to
the genus 'mycobacterium', which include the causative agent for
tuberculosis.
PRINCIPLE: The lipoid capsule of the acid-fast organism takes up carbolfuchsin
and resists decolorization with a dilute acid rinse. The lipoid
capsule of the mycobacteria is of such high molecular weight that it is
waxy at room temperature and successful penetration by the aqueousbased
staining solutions (such as Gram's) is prevented.
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