A-bile solubility test


this test used to differentiate s.pneumonia,which is soluble in bile and

bile salt,from viridans streptococci which are insoluble
b -catalase test
this test is used to differentiate those bacteria that produce the enzyme catalase such as staphylococci,from non-catalase producing bacteria such as streptococci
c -coagulase test
this test is used to differentiate s.aureus which produces the enzyme coagulase,from s.epidermidis ands.saprophyticus whichdo not produce coagulase
d -citrate utilization test
this test used to assist in identification of enterobacteriacea
this test based on the ability of an organism to use citrate as its only source of carbon and ammonia as its only source of nitrogen
e -deoxy ribonuclease(DNAse)test
aiso used to differntiate s.aureus which produce the enzyme DNAse
it is particularly useful if plasma is not available to perform acoagulase test or when the results of acoagulase test are difficult to interpret
f -hydrogen sulphide(H2S)Test
the detection of hydrogen sulphid gas is used mainly to assist in the identification of enterbacteria and to differentiate other bacteria such as Bacteroides and Brucella species
g-indole test
this test used in identification of enterobacteria
most strains of E.coli,P.vulgaris,and providencia break down the amino acid tryptophan with the release of indole
h -litmus milk decolorization test
this test assist in the identification of enterococcito reduce litmus milk by enzyme action
i -nitrate reduction test
this test used to differentiate members of enterobacteriacea that produce the enzyme nitrate reductase,from gram negative bacteria that do not produce the enzyme
aiso is helpfulin differentiatig mycobacterium species
j -oxidase test
this test is used to assist in the identification of pseudomonas,neisseria,vibrio,and pasteurella species,all of which produce oxidase enzymes
k -oxidation-fermentation(O-F)test
this test is used to differentiate those organisms that oxidize carbohydrates
,aerobic utilization such aspseudomonase aeruginosa,from thos organisms that ferment carbohydrates
(anaerobic utilization ) such as members of enterobacteriaceae
l-TWEEN 80 HYDROLYSIS TEST
used to differentiate slow-growing mycobacterium species
hydrolize the detergent Tween 80 with production of oleic acid
m-urease test
testing for urease enzyme activity is important in differentiating enterobacteria.proteus strains are strong urease producers,y.enterocolitica also shows urease activity,but salmonella and shigellae do not produce urease
n -arylsulphatase test
this test used to differntiate mycobacterium species
o-quellung reaction
used to determine the specific capsular type arround the bacteria