تويت
Plasma Total Protein by Biuret method
* on clean and clear test tube *
add 1 ml >> Biuret reagent
add 0.02 ml ( 20 micro L ) >> pt sample
Mix will
incubate at 10 min at room temp ( 25 C ) or at 37 c for 5 min .. and wait
the final color converted from BLUE ( origenal ) to VIOLET or PERPLE color releated to protein concenterated in plasma
read test sample and standard solution againist BLANK .. by use filter 580 nm
read by COLORIMETER or SPECTROPHOTOMETER
* RESULT :
optecal dansity of test / optical dansity of std * conc of std ( 6 - 7 - 8 g/dl ) 60 or 70 or 80 g/l
* NORMAL RANGE - REFERANE VALUE :
old unit 6 - 8 g / dl
SI unit 60 - 80 g / l
or depend on condition of area
* COMMENT :
report as : HYPER PROTEINAEMIA or HYPO PROTEINAEMIA or NORMAL PROTEINAEMIA
ولو في مصطلح أو اختصار ماهو واضح فأنا جاهز للتوضيح وغيري في القسم إنشاء الله أبخص with best wishes
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