تويت
CAMPYLOBACTER
Campylobacter
الخصائص العامة
1-Gram- negative curved rods
2-motile with uni or bi-polar flagella
3)-charactarized by darting motality under dark field microscopy
4)-charactarized by corkscrew apperance
5)-consider microaerophilic bacteria
1-Gram- negative curved rods
2-motile with uni or bi-polar flagella
3)-charactarized by darting motality under dark field microscopy
4)-charactarized by corkscrew apperance
5)-consider microaerophilic bacteria
What is microerophilic bacteria
specifice types of bacteria require oxygen to surviving but requires enviroments containig lower levels of oxygen than are present in the atmosphere
represent (3-10 %) of oxygen
so they can be easily cultured in acandel jar
6)- non-spore forming
species
campylobacter have 16 species and 6 sub-species but the common is
a)-campylobacter.jejuni
consider the first number in food poisoning
colonies in the gastrointestinal tract pf birds
produce cytodistending toxin
represent (3-10 %) of oxygen
so they can be easily cultured in acandel jar
6)- non-spore forming
species
campylobacter have 16 species and 6 sub-species but the common is
a)-campylobacter.jejuni
consider the first number in food poisoning
colonies in the gastrointestinal tract pf birds
produce cytodistending toxin
campylobacter.jejuni
b)-campylobacter.fetus
causes a spontaneous abortion in cattel and sheep
c)-campylobacter.coli
Temperature that required to growth is 42C
causes a spontaneous abortion in cattel and sheep
c)-campylobacter.coli
Temperature that required to growth is 42C
LABORATORY DIAGNOSIS
stool culture used to identify the campylobacter bacteria
media used in diagnosis
this media is selective media
a-)campy-blood agar
Brucella agar suplemented with sheep blood and vancomycin(to inhibit gram- positive bacteria),trimethoprim(broad spectrum)and cephalothin to inhibit streptococci
b-)skirrow,s agar
peptone and soy protein base agar suplemented with lysed horse blood with
vancomycin to inhibit gram-positiv
trimethoprim broad spectrum and
polymixin B as antifungal
c-)Butzler agar
blood agar prepared from colombia agar
media used in diagnosis
this media is selective media
a-)campy-blood agar
Brucella agar suplemented with sheep blood and vancomycin(to inhibit gram- positive bacteria),trimethoprim(broad spectrum)and cephalothin to inhibit streptococci
b-)skirrow,s agar
peptone and soy protein base agar suplemented with lysed horse blood with
vancomycin to inhibit gram-positiv
trimethoprim broad spectrum and
polymixin B as antifungal
c-)Butzler agar
blood agar prepared from colombia agar
Requirment required for isolation
a-)large sample size
b-)selective enrichment broth
c-)suitable microaerphilic condition
methods of isolation of campylobacter
a-)large sample size
b-)selective enrichment broth
c-)suitable microaerphilic condition
methods of isolation of campylobacter
they are tow methods
A-)Filtration technique
A-)Filtration technique
- suspend the required amount of sample or its rins in a suitable amount of diluted buffer
- centrifuge at 650-800 rpm for 10 minutes
- collect the supernatant
- pass the supernatant through two 25 mm filter chamber
aded 2-4 drops of the filtrate are spread on chocolate agar and incubated under microaerophilic enviroment
b-)selective Broth Enrichment and plating
Doyle and Roman enrichment broth consist of Brucella broth with added antibiotic suplement is inoculated with 10 to 25 gram sample,and incubated with agitation under microaerophilic atmospher at 42C for 16-18 hours
the enrichment culture is plated on selective media, and the plates are incubated at the same condition as an enrichment broth with no agitation required
Biochemical test
a-)oxidase ---------positive
b-)catalase-------positive
c-)nitrate reduction--------- positive
d-)glucose utilization--------negative
g-)Hippurate hydrolysis------- positive
Hippurate is organic compound found in the urine of horses and other herbivores
h-)Nalidixic acid--------sensetive
i-)cephalothoin test------ resistant
b-)selective Broth Enrichment and plating
Doyle and Roman enrichment broth consist of Brucella broth with added antibiotic suplement is inoculated with 10 to 25 gram sample,and incubated with agitation under microaerophilic atmospher at 42C for 16-18 hours
the enrichment culture is plated on selective media, and the plates are incubated at the same condition as an enrichment broth with no agitation required
Biochemical test
a-)oxidase ---------positive
b-)catalase-------positive
c-)nitrate reduction--------- positive
d-)glucose utilization--------negative
g-)Hippurate hydrolysis------- positive
Hippurate is organic compound found in the urine of horses and other herbivores
h-)Nalidixic acid--------sensetive
i-)cephalothoin test------ resistant
PATHOGENISIS
campylobacter causes the gastroenteritis inhuman especially in children
symptoms appear after 2-5 days of infection
symptoms
symptoms appear after 2-5 days of infection
symptoms
- dirrhea
- abdomin pain
- fever
- cramps
- headache
- bloody stool
- dehydration
Transmistion
campylobacter transmitted from animals and animals products to human
- oral-fecal
- contaminated food and water
- eating of raw meat
- prson to person sexual contact
Treatment
most campylobacteriosis will recover without medication ,but in sever cases especially in children take antibiotices as
ciprofloxin and erthromycin
fluids and electrolytesmay be required for serious cases
ciprofloxin and erthromycin
fluids and electrolytesmay be required for serious cases
prevention
a-)kill any bacteria in meat by good cooking
b-)test water befor drinking
c-)pasterulization of milk
d-)wash your hands befor and after touching Raw meat espically poultry
b-)test water befor drinking
c-)pasterulization of milk
d-)wash your hands befor and after touching Raw meat espically poultry
اسعد اللة صباحكم بخير
اتمنى ان تعم الفائدة على الجميع
دمتم فى رعاية اللة وحفظة
اتمنى ان تعم الفائدة على الجميع
دمتم فى رعاية اللة وحفظة
تعليق