بسم الله الرحمن الرحيم
citrate utilization test
bacteria use Na citrate as asource of carbon
Media: simmons ajar media "solid media"
normally green color in positive result convert into blue color
bromothymol blue whitch is blue color in alkaline PHwhitch is produced by utilization of citrate
EXositive result salmonella
negative result E.Coli
عبدالحميد فارس
ممكن حد يوضحلى كيفية وضع الصور عشان مش عارف شكرا
]EMB
used to differentiate between lactose fermenter and non lactose fermenter especially E Coli
selective agent is eosin methyline blue
differentiate between pathogenic and non pathogenic E Coli
pathogenic E Coli give greenish color colonies with metalic sheen
nonpathogenic E Coli give greenish color colonies without metalic sheen
Eosin methylene blue agar, as the name suggests, contains the dyes eosin and methylene blue.
EMB agar is selective because the aniline dyes in this purple media inhibit growth of Gram-positive organisms.
Lactose fermenters metabolize the lactose in the media and produce acid byproducts, causing a color change in the colony.
Thus, EMB is also a differential medium.
Strong acid production by organisms such as E. coli
results in a metallic green sheen like the picture in the question.
Weaker fermentation of lactose results in
colonies with a pinkish-purple color.
Colonies of nonlactose fermenters remain colorless,
or at least are no darker than the color of the media.
]EMB
used to differentiate between lactose fermenter and non lactose fermenter especially E Coli
selective agent is eosin methyline blue
differentiate between pathogenic and non pathogenic E Coli
pathogenic E Coli give greenish color colonies with metalic sheen
nonpathogenic E Coli give greenish color colonies without metalic sheen
EMB agar are selective and differential media
selective due to presence of selective agaent such as eosin and methyline blue that prevent growth of G-positive bacteria
differential differentiate between enteric G-negative bacteria differentiate between lactose fermenter and non-lactose fermenter
شكرا اخى عبد الحميد فارس من المحروسة ام الدنيا مصر
Eosin methylene blue agar, as the name suggests, contains the dyes eosin and methylene blue. EMB agar is selective because the aniline dyes in this purple media inhibit growth of Gram-positive organisms. Lactose fermenters metabolize the lactose in the media and produce acid byproducts, causing a color change in the colony. Thus, EMB is also a differential medium. Strong acid production by organisms such as E. coli
results in a metallic green sheen like the picture in the question. Weaker fermentation of lactose results in colonies with a pinkish-purple color.
Colonies of nonlactose fermenters remain colorless, or at least are no darker than the color of the media.
the green metalic sheen appear due to precipitation of methelyne blue in the media from the very high amount of acid production from fermentation
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