coagulase test is performed to diffrentiate bitween the staph.arues & others of staphelococci group.
we used two methods:
slide method (bound mehod)
test tube method (free method)
slide method
put two drops of distill water in each end of slide.
by sterile wire loop take good growth of organism and make a suspention with the two drops on the slide.
add the plasma (few 1drop) at one end of slide not both.
observe the clottin under microscope.
result:
clotting---- +ve (presence of staph.arues)
no clotting----- -ve ( spieces of staph other than aureus)
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